| 28 | Brian Baker | P | AAA | 6-5 | 190 | Right | Right | 1/10/1983 | 23 | Brooklyn,New York | 2005 | UDFA | | TB |
|
| 35 | Josh Butler | P | MLB | 6-5 | 195 | Right | Right | 12/11/1984 | 21 | Auburn,California | 2006 | 2 | 47 | TB |
| 4 | Joey Callender | 2B | A+ | 5-11 | 165 | Right | Right | 11/25/1983 | 22 | Bossier City,Louisiana | 2006 | 21 | 619 | TB |
| 7 | Jairo De la Rosa | 3B | A+ | 6-2 | 170 | Right | Right | 9/8/1985 | 20 | Santo Domingo,Dominican Republic | 2004 | UDFA | | TB |
| - | Seth Dhaenens | 2B | A | 6-2 | 175 | Left | Right | 5/20/1984 | 22 | Mesa,Arizona | 2002 | 50 | 1471 | BOS |
| 6 | Eric Durante | 2B | A | 5-10 | 175 | Right | Right | 7/11/1984 | 21 | Honolulu,Hawaii | 2005 | UDFA | | TB |
| 26 | Matt Falk | IF-P | A | 6-3 | 190 | Left | Right | 8/25/1984 | 21 | San Juan Capistrano,California | 2005 | 28 | 838 | TB |
| 37 | Matt Fields | 1B | AA | 6-4 | 255 | Right | Right | 7/8/1985 | 20 | Seattle,Washington | 2004 | 44 | 1302 | TB |
| 27 | Woods Fines | P | A | 6-5 | 190 | Right | Right | 8/14/1985 | 20 | Chapel Hill,North Carolina | 2004 | 14 | 405 | TB |
| 16 | Neal Frontz | P | AA | 6-3 | 190 | Right | Right | 4/6/1984 | 22 | Fort Worth,Texas | 2005 | 24 | 718 | TB |
| 14 | Roberto Gil | P | A- | 6-1 | 160 | Right | Right | 5/20/1984 | 22 | San Pedro de Macoris,Dominican Republic | 2004 | UDFA | | TB |
| 34 | Scott Gummo | 1B | A- | 6-1 | 230 | Right | Right | 9/22/1982 | 23 | Altoona,Pennsylvania | 2006 | UDFA | | TB |
| 18 | Josh Hamilton | CF-LF | MLB | 6-4 | 240 | Left | Left | 5/21/1981 | 25 | Raleigh,North Carolina | 1999 | 1 | 1 | TB |
| 24 | Jeremy Hellickson | P | MLB | 6-1 | 185 | Right | Right | 4/8/1987 | 19 | Des Moines,Iowa | 2005 | 4 | 118 | TB |
| 44 | David Kennedy | OF | A- | 6-2 | 178 | Both | Right | 7/10/1983 | 22 | Moreno Valley,California | 2005 | UDFA | | TB |
| 13 | Thomas Lagreid | C | A | 6-1 | 185 | Right | Right | 10/24/1983 | 22 | Edmonds,Washington | 2003 | 31 | 908 | TB |
| 19 | Matt Larson | P | A | 6-4 | 215 | Right | Right | 4/9/1981 | 25 | Newport Beach,California | 2004 | UDFA | | TB |
| - | Evan Longoria | 3B | MLB | 6-2 | 180 | Right | Right | 10/7/1985 | 20 | Downey,California | 2006 | 1 | 3 | TB |
| 9 | Maiko Loyola | OF | A+ | 5-11 | 150 | Right | Right | 7/19/1985 | 20 | San Cristobal,Dominican Republic | 2002 | UDFA | | PIT |
| 12 | John Matulia | RF-CF | AAA | 6-0 | 175 | Left | Left | 8/19/1986 | 19 | Thomaston,Georgia | 2005 | 10 | 298 | TB |
| 5 | James Mayer | SS | A | 5-11 | 165 | Right | Right | 3/8/1984 | 22 | Johnston,Pennsylvania | 2006 | 30 | 889 | TB |
| - | Ryan Morse | P | AA | 6-4 | 205 | Left | Left | 5/21/1983 | 23 | St. Cloud,Minnesota | 2005 | 13 | 388 | TB |
| 33 | Joseph Muro | P | A- | 6-0 | 161 | Right | Right | 2/16/1984 | 22 | La Mirada,California | 2004 | 9 | 255 | TB |
| 25 | Brett Nagy | P | A- | 6-1 | 190 | Right | Right | 11/10/1981 | 24 | Jacksonville,Florida | 2005 | UDFA | | TB |
| 32 | Ryan Owen | P | A+ | 6-4 | 200 | Left | Left | 7/26/1984 | 21 | San Dimas,California | 2006 | 16 | 469 | TB |
| 29 | Ian Paxton | C | AA | 6-1 | 210 | Right | Right | 9/4/1983 | 22 | Springfield,Missouri | 2006 | UDFA | | TB |
| 36 | Ryan Reid | P | AAA | 6-0 | 215 | Left | Right | 4/24/1985 | 21 | Portland,Maine | 2006 | 7 | 199 | TB |
| - | Claudio Rodriguez | P | A+ | 6-5 | 220 | Right | Right | 6/29/1983 | 23 | Bonao,Dominican Republic | 2002 | UDFA | | TB |
| 30 | Heath Rollins | P | AAA | 6-1 | 190 | Right | Right | 5/25/1985 | 21 | Florence,South Carolina | 2006 | 11 | 319 | TB |
| 39 | Celso Rondon | P | A | 6-0 | 160 | Right | Right | 4/7/1984 | 22 | Cumana,Venezuela | 2001 | UDFA | | NYM |
| 8 | Brandon Rousseve | SS | A | 6-1 | 175 | Right | Right | 8/4/1984 | 21 | New Orleans,Louisiana | 2003 | 45 | 1322 | TB |
| 21 | Ryan Royster | OF | AA | 6-2 | 205 | Right | Right | 7/25/1986 | 19 | Eugene,Oregon | 2004 | 6 | 165 | TB |
| - | Ben Rulon | P | A- | 6-5 | 210 | Left | Left | 7/4/1985 | 20 | Roseville,Illinois | 2005 | 38 | 1138 | TB |
| 41 | Matt Spring | C | AAA | 6-2 | 215 | Right | Right | 11/7/1984 | 21 | Phoenix,Arizona | 2004 | 4 | 105 | TB |
| 31 | Quinn Stewart | OF | A+ | 6-1 | 200 | Right | Right | 8/28/1983 | 22 | Rowlett,Texas | 2006 | UDFA | | TB |
| 40 | Erik Walker | P | A- | 6-4 | 225 | Left | Right | 10/15/1983 | 22 | Clemmons,North Carolina | 2006 | 20 | 589 | TB |