| # | Player Name | Pos | Age | B | T | Ht | Wt | Born | Place | High Level | mlbYears | Salary |
| 41 | Alberto Arias | P | 24 | R | R | 5-11 | 150 | 10/14/1983 | Santo Domingo,Dominican Republic | MLB | 2007-2009 | min |
| 27 | Garrett Atkins | 3B | 28 | R | R | 6-3 | 210 | 12/12/1979 | Irvine,California | MLB | 2003-2010 | 4,387,500 |
| 10 | Jeff Baker | 3B | 27 | R | R | 6-2 | 210 | 6/21/1981 | Badkissagen,Germany | MLB | 2005-2011 | 403,000 |
| 21 | Clint Barmes | SS-2B | 29 | R | R | 6-1 | 190 | 3/6/1979 | Vincennes,Indiana | MLB | 2003-2011 | 405,000 |
| 15 | Edwin Bellorin | C | 26 | R | R | 5-11 | 170 | 2/21/1982 | Edo Bolivar,Venezuela | MLB | 2007-2009 | min |
| 1 | Doug Bernier | IF | 28 | B | R | 5-11 | 175 | 6/24/1980 | Santa Maria,California | MLB | 2008-2008 | min |
| 31 | Cedrick Bowers | P | 30 | R | L | 6-2 | 223 | 2/10/1978 | Gainesville,Florida | MLB | 2008-2010 | min |
| 44 | Micah Bowie | P | 33 | L | L | 6-4 | 210 | 11/10/1974 | Webster,Texas | MLB | 1999-2008 | 650,000 |
| 35 | Taylor Buchholz | P | 26 | R | R | 6-4 | 220 | 10/13/1981 | Lower Merion,Pennsylvania | MLB | 2006-2011 | 399,000 |
| 41 | Jose Capellan | P | 27 | R | R | 6-3 | 170 | 1/13/1981 | Cotui,Dominican Republic | MLB | 2004-2008 | min |
| 28 | Aaron Cook | P | 29 | R | R | 6-3 | 175 | 2/8/1979 | Fort Campbell,Kentucky | MLB | 2002-2011 | 5,125,000 |
| 60 | Manuel Corpas | P | 25 | R | R | 6-3 | 170 | 12/3/1982 | Panama City,Panama | MLB | 2006-2010 | 775,000 |
| 29 | Jorge De La Rosa | P | 27 | L | L | 6-1 | 190 | 4/5/1981 | Monterrey,Mexico | MLB | 2004-2011 | min |
| 39 | Valerio De Los Santos | P | 32 | L | L | 6-2 | 180 | 10/6/1975 | Las Matas de Farfan,Dominican Republic | MLB | 1998-2008 | min |
| 14 | Dexter Fowler | OF | 22 | B | R | 6-5 | 189 | 3/22/1986 | Alpharetta,Georgia | MLB | 2008-2011 | min |
| 26 | Jeff Francis | P | 27 | L | L | 6-5 | 200 | 1/8/1981 | Vancouver,British Columbia | MLB | 2004-2011 | 3,000,000 |
| 40 | Brian Fuentes | P | 32 | L | L | 6-4 | 220 | 8/9/1975 | Merced,California | MLB | 2001-2011 | 5,050,000 |
| 49 | Jason Grilli | P | 31 | R | R | 6-4 | 185 | 11/11/1976 | Royal Oak,Michigan | MLB | 2000-2011 | 420,000 |
| 11 | Brad Hawpe | RF | 29 | L | L | 6-3 | 210 | 6/22/1979 | Fort Worth,Texas | MLB | 2004-2011 | 3,925,000 |
| 17 | Todd Helton | 1B | 34 | L | L | 6-2 | 206 | 8/20/1973 | Knoxville,Tennessee | MLB | 1997-2011 | 16,600,000 |
| 34 | Matt Herges | P | 38 | L | R | 6-0 | 200 | 4/1/1970 | Champaign,Illinois | MLB | 1999-2009 | 2,000,000 |
| 61 | Livan Hernandez | P | 33 | R | R | 6-2 | 225 | 2/20/1975 | Villa Clara,Cuba | MLB | 1996-2011 | 5,000,000 |
| 21 | Jonathan Herrera | 2B | 23 | B | R | 5-9 | 150 | 11/3/1984 | Maracaibo,Venezuela | MLB | 2008-2011 | min |
| 48 | Jason Hirsh | P | 26 | R | R | 6-8 | 250 | 2/20/1982 | Santa Monica,California | MLB | 2006-2008 | 396,000 |
| 5 | Matt Holliday | LF | 28 | R | R | 6-4 | 235 | 1/1/1980 | Stillwater,Oklahoma | MLB | 2004-2011 | 9,500,000 |
| 20 | Chris Iannetta | C | 25 | R | R | 5-11 | 195 | 4/8/1983 | Providence,Rhode Island | MLB | 2006-2011 | 396,000 |
| 38 | Ubaldo Jimenez | P | 24 | R | R | 6-2 | 160 | 1/22/1984 | Nagua,Dominican Republic | MLB | 2006-2011 | 392,000 |
| 47 | Joe Koshansky | P-1B | 26 | L | L | 6-4 | 225 | 5/26/1982 | Shreveport,Louisiana | MLB | 2007-2008 | min |
| 16 | Adam Melhuse | C | 36 | B | R | 6-2 | 185 | 3/27/1972 | Santa Clara,California | MLB | 2000-2008 | 750,000 |
| 56 | Franklin Morales | P | 22 | L | L | 6-0 | 170 | 1/24/1986 | San Juan Morros,Venezuela | MLB | 2007-2011 | 391,000 |
| 52 | Juan Morillo | P | 24 | R | R | 6-1 | 160 | 11/5/1983 | San Pedro de Macoris,Dominican Republic | MLB | 2006-2009 | min |
| 45 | Josh Newman | P | 26 | L | L | 6-1 | 215 | 6/11/1982 | Wheelersburg,Ohio | MLB | 2007-2008 | min |
| 1 | Jayson Nix | 2B | 25 | R | R | 5-11 | 180 | 8/26/1982 | Dallas,Texas | MLB | 2008-2011 | 390,000 |
| 29,22 | Scott Podsednik | CF | 32 | L | L | 6-0 | 170 | 3/18/1976 | West,Texas | MLB | 2001-2010 | 750,000 |
| 6 | Omar Quintanilla | SS | 26 | L | R | 5-9 | 185 | 10/24/1981 | El Paso,Texas | MLB | 2005-2011 | min |
| 55 | Mark Redman | P | 34 | L | L | 6-5 | 220 | 1/5/1974 | San Diego,California | MLB | 1999-2008 | 1,000,000 |
| 41 | Steve Register | P | 25 | R | R | 6-1 | 170 | 5/16/1983 | Columbus,Georgia | MLB | 2008-2009 | min |
| 54 | Greg Reynolds | P | 22 | R | R | 6-7 | 225 | 7/3/1985 | Pacifica,California | MLB | 2008-2011 | min |
| 32 | Glendon Rusch | P | 33 | L | L | 6-1 | 200 | 11/7/1974 | Seattle,Washington | MLB | 1997-2009 | 850,000 |
| 25 | Seth Smith | OF | 25 | L | L | 6-3 | 215 | 9/30/1982 | Beverly Hills,California | MLB | 2007-2011 | min |
| 23 | Ryan Speier | P | 28 | R | R | 6-7 | 200 | 7/24/1979 | Frankfort,Kentucky | MLB | 2005-2009 | min |
| 19 | Ryan Spilborghs | OF | 28 | R | R | 6-1 | 190 | 9/5/1979 | Santa Barbara,California | MLB | 2005-2011 | 396,000 |
| 24 | Ian Stewart | 3B | 23 | L | R | 6-3 | 200 | 4/5/1985 | Long Beach,California | MLB | 2007-2011 | min |
| 18 | Cory Sullivan | OF | 28 | L | L | 6-0 | 180 | 8/20/1979 | Tulsa,Oklahoma | MLB | 2005-2010 | min |
| 3 | Willy Taveras | CF-LF | 26 | R | R | 6-0 | 160 | 12/25/1981 | Tenares,Dominican Republic | MLB | 2004-2010 | 1,975,000 |
| 8 | Yorvit Torrealba | C | 29 | R | R | 5-11 | 180 | 7/19/1978 | Caracas,Venezuela | MLB | 2001-2011 | 3,000,000 |
| 2 | Troy Tulowitzki | SS | 23 | R | R | 6-3 | 205 | 10/10/1984 | Santa Clara,California | MLB | 2006-2011 | 750,000 |
| 51 | Luis Vizcaino | P | 31 | R | R | 5-11 | 169 | 6/1/1977 | Bani,Dominican Republic | MLB | 1999-2009 | 3,500,000 |
| 16 | Kip Wells | P | 31 | R | R | 6-3 | 196 | 4/21/1977 | Houston,Texas | MLB | 1999-2009 | 3,100,000 |