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| 1. | David Price | | P | Tampa Bay Devil Rays | 21 | 8/26/1985 | 6-6 | 215 | L | L | College | | Vanderbilt University | 87 |
| 2. | Mike Moustakas | | 3B | Kansas City Royals | 18 | 9/11/1988 | 6-0 | 195 | L | R | Rookie | | Chatsworth High School (Chatsworth,CA) | 20 |
| 3. | Josh Vitters | | 3B | Chicago Cubs | 17 | 8/27/1989 | 6-3 | 200 | R | R | A- | | Cypress High School (Cypress,CA) | 8 |
| 4. | Daniel Moskos | | P | Pittsburgh Pirates | 21 | 4/28/1986 | 6-1 | 210 | R | L | A- | | Clemson University | 175 |
| 5. | Matt Wieters | | C | Baltimore Orioles | 21 | 5/21/1986 | 6-4 | 225 | S | R | College | | Georgia Tech | 133 |
| 6. | Ross Detwiler | | P | Washington Nationals | 21 | 3/6/1986 | 6-5 | 185 | R | L | MLB | 2007-2007 | Southwest Missouri State University | 73 |
| 7. | Matt LaPorta | | 1B | Milwaukee Brewers | 22 | 1/8/1985 | 6-2 | 212 | R | R | A | | University of Florida | 143 |
| 8. | Casey Weathers | | P | Colorado Rockies | 22 | 6/10/1985 | 6-1 | 205 | R | R | A+ | | Vanderbilt University | 88 |
| 9. | Jarrod Parker | | P | Arizona Diamondbacks | 18 | 11/24/1988 | 6-1 | 180 | R | R | - | | Norwell High School (Ossian,IN) | 3 |
| 10. | Madison Bumgarner | | P | San Francisco Giants | 17 | 8/1/1989 | 6-4 | 215 | R | L | - | | South Caldwell High School (Hudson,NC) | 1 |
| 11. | Phillippe Aumont | | P | Seattle Mariners | 18 | 1/7/1989 | 6-7 | 220 | L | R | - | | Ecole du Versant High School (Gatineau,QC) | |
| 12. | Matt Dominguez | | 3B | Florida Marlins | 17 | 8/28/1989 | 6-2 | | R | R | A- | | Chatsworth High School (Chatsworth,CA) | 21 |
| 13. | Beau Mills | | 3B/1B | Cleveland Indians | 20 | 8/15/1986 | 6-3 | 220 | L | R | A+ | | Lewis-Clark State College | 88 |
| 14. | Jason Heyward | | OF | Atlanta Braves | 17 | 8/9/1989 | 6-4 | 220 | L | L | Rookie | | Henry County High School (McDonough,GA) | 5 |
| 15. | Devin Mesoraco | | C | Cincinnati Reds | 19 | 6/19/1988 | 6-1 | | R | R | Rookie | | Punxsotawney High School (Punxsotawney,PA) | 2 |
| 16. | Kevin Ahrens | | 3B | Toronto Blue Jays | 18 | 4/26/1989 | 6-1 | 190 | S | R | Rookie | | Memorial Senior High School (Houston,TX) | 4 |
| 17. | Blake Beavan | | P | Texas Rangers | 18 | 1/17/1989 | 6-6 | 200 | R | R | - | | Irving High School (Irving,TX) | 3 |
| 18. | Pete Kozma | | SS | St. Louis Cardinals | 19 | 4/11/1988 | 6-0 | 170 | R | R | A- | | Owasso High School (Owasso,OK) | 7 |
| 19. | Joe Savery | | P | Philadelphia Phillies | 21 | 11/4/1985 | 6-3 | 215 | L | L | A- | | Rice University | 116 |
| 20. | Chris Withrow | | P | Los Angeles Dodgers | 18 | 4/1/1989 | 6-3 | 195 | R | R | Rookie | | Midland High School (Midland,TX) | 7 |
| 21. | J.P. Arencibia | | C/1B | Toronto Blue Jays | 21 | 1/5/1986 | 6-1 | 210 | R | R | A- | | University of Tennessee | 114 |
| 22. | Tim Alderson | | P | San Francisco Giants | 18 | 11/3/1988 | 6-6 | 217 | R | R | Rookie | | Horizon High School (Scottsdale,AZ) | 18 |
| 23. | Nick Schmidt | | P | San Diego Padres | 21 | 10/10/1985 | 6-5 | 220 | S | L | A | | University of Arkansas | 147 |
| 24. | Michael Main | $$ | P/OF | Texas Rangers | 18 | 12/14/1988 | 6-2 | 170 | R | R | A- | | Deland High School (Deland,FL) | 8 |
| 25. | Aaron Poreda | | P | Chicago White Sox | 20 | 10/1/1986 | 6-6 | 240 | L | L | Rookie | | University of San Francisco | 59 |
| 26. | James Simmons | $$ | P | Oakland Athletics | 20 | 9/29/1986 | 6-3 | 205 | R | R | AA | | University of California-Riverside | 89 |
| 27. | Rick Porcello | | P | Detroit Tigers | 18 | 12/27/1988 | 6-5 | 195 | R | R | - | | Seton Hall Prep High School (West Orange,NJ) | 5 |
| 28. | Ben Revere | | OF | Minnesota Twins | 19 | 5/3/1988 | 5-9 | 165 | L | R | Rookie | | Lexington Catholic High School (Lexington,KY) | 3 |
| 29. | Wendell Fairley | | OF | San Francisco Giants | 19 | 3/17/1988 | 6-2 | 190 | L | R | - | | George County High School (Lucedale,MS) | 3 |
| 30. | Andrew Brackman | | P | New York Yankees | 21 | 12/4/1985 | 6-11 | 230 | R | R | College | | North Carolina State University | 100 |
| 31. | Josh Smoker | | P | Washington Nationals | 18 | 11/26/1988 | 6-2 | 195 | L | L | A- | | Calhoun High School (Calhoun,GA) | 4 |
| 32. | Nick Noonan | | 2B | San Francisco Giants | 18 | 5/4/1989 | 6-0 | 180 | L | R | Rookie | | Parker High School (San Diego,CA) | |
| 33. | Jon Gilmore | | 3B | Atlanta Braves | 18 | 8/23/1988 | 6-3 | | R | R | Rookie | | Iowa City High School (Iowa City,IA) | 3 |
| 34. | Todd Frazier | | 3B | Cincinnati Reds | 21 | 2/12/1986 | 6-3 | 215 | R | R | A | | Rutgers University | 61 |
| 35. | Julio Borbon | | OF | Texas Rangers | 21 | 2/20/1986 | 6-1 | 180 | L | L | A- | | University of Tennessee | 115 |
| 36. | Clayton Mortensen | | P | St. Louis Cardinals | 22 | 4/10/1985 | 6-4 | 180 | R | R | A | | Gonzaga University | 58 |
| 37. | Travis D'Arnaud | | C | Philadelphia Phillies | 18 | 2/10/1989 | 6-2 | 195 | R | R | Rookie | | Lakewood High School (Lakewood,CA) | 47 |
| 38. | Brett Cecil | | P | Toronto Blue Jays | 20 | 7/2/1986 | 6-3 | 220 | R | L | A- | | University of Maryland | 75 |
| 39. | James Adkins | | P | Los Angeles Dodgers | 21 | 11/26/1985 | 6-5 | 195 | L | L | A | | University of Tennessee | 116 |
| 40. | Kellen Kulbacki | | OF | San Diego Padres | 21 | 11/21/1985 | 5-11 | 185 | L | L | A- | | James Madison University | 67 |
| 41. | Sean Doolittle | | 1B/P | Oakland Athletics | 20 | 9/26/1986 | 6-3 | 190 | L | L | A+ | | University of Virginia | 62 |
| 42. | Eddie Kunz | | P | New York Mets | 21 | 4/8/1986 | 6-5 | 250 | R | R | A- | | Oregon State University | 94 |
| 43. | Jackson Williams | | C | San Francisco Giants | 21 | 5/14/1986 | 5-11 | 200 | R | R | A- | | University of Oklahoma | 202 |
| 44. | Neil Ramirez | | P | Texas Rangers | 18 | 5/25/1989 | 6-3 | 185 | R | R | - | | Kempsville High School (Virginia Beach,VA) | 3 |
| 45. | Justin Jackson | | SS | Toronto Blue Jays | 18 | 12/11/1988 | 6-2 | 175 | R | R | Rookie | | Roberson Skyland High School (Asheville,NC) | 2 |
| 46. | Drew Cumberland | | SS | San Diego Padres | 18 | 1/13/1989 | 5-10 | 175 | L | R | A- | | Pace High School (Pace,FL) | 1 |
| 47. | Nathan Vineyard | | P | New York Mets | 18 | 10/3/1988 | 6-2 | 200 | L | L | Rookie | | Woodland High School (Cartersville,GA) | |
| 48. | Josh Donaldson | | C | Chicago Cubs | 21 | 12/8/1985 | 6-0 | 195 | R | R | A- | | Auburn University | 126 |
| 49. | Michael Burgess | | OF | Washington Nationals | 18 | 10/20/1988 | 5-11 | 195 | L | L | A- | | Hillsborough High School (Tampa,FL) | 38 |
| 50. | Wes Roemer | | P | Arizona Diamondbacks | 20 | 10/7/1986 | 6-0 | 190 | R | R | A- | | California State University, Fullerton | 234 |
| 51. | Charlie Culberson | | 2B/SS | San Francisco Giants | 18 | 4/10/1989 | 6-1 | 185 | R | R | Rookie | | Calhoun High School (Calhoun,GA) | 5 |
| 52. | Matt Mangini | | 3B | Seattle Mariners | 21 | 12/21/1985 | 6-4 | 220 | L | R | A+ | | Oklahoma State University | 185 |
| 53. | Kyle Lotzkar | | P | Cincinnati Reds | 17 | 10/24/1989 | 6-4 | 200 | L | R | Rookie | | South Delta High School (Delta,BC) | |
| 54. | Tommy Hunter | | P | Texas Rangers | 20 | 7/3/1986 | 6-3 | 255 | R | R | A- | | University of Alabama | 111 |
| 55. | Nick Hagadone | | P | Boston Red Sox | 21 | 1/1/1986 | 6-5 | 230 | L | L | A- | | University of Washington | 103 |
| 56. | Trystan Magnuson | | P | Toronto Blue Jays | 22 | 6/6/1985 | 6-7 | 210 | L | R | College | | University of Louisville | 25 |
| 57. | Mitch Canham | | C | San Diego Padres | 22 | 9/25/1984 | 6-2 | 215 | L | R | A+ | | Oregon State University | 95 |
| 58. | Jonathan Bachanov | | P | Los Angeles Angels | 18 | 1/30/1989 | 6-4 | 210 | R | R | - | | University High School (Orlando,FL) | 2 |
| 59. | Corey Brown | | OF | Oakland Athletics | 21 | 11/26/1985 | 6-2 | 210 | L | L | A- | | Oklahoma State University | 186 |
| 60. | Brandon Hamilton | | P | Detroit Tigers | 18 | 12/25/1988 | 6-2 | 205 | R | R | - | | Stanhope Elmore High School (Millbrook,AL) | 7 |
| 61. | Ed Easley | | C | Arizona Diamondbacks | 21 | 12/21/1985 | 6-1 | 180 | R | R | A- | | Mississippi State University | 141 |
| 62. | Ryan Dent | | SS/2B | Boston Red Sox | 18 | 3/15/1989 | 6-0 | 190 | R | R | A- | | Wilson High School (Long Beach,CA) | 14 |
| 63. | Cory Luebke | | P | San Diego Padres | 22 | 3/4/1985 | 6-4 | 200 | R | L | A+ | | Ohio State University | 96 |
| 64. | Danny Payne | | OF | San Diego Padres | 21 | 9/8/1985 | 5-10 | 185 | L | L | A | | Georgia Tech | 134 |
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