| # | Player Name | Pos | Team | Age | Born | Ht | Wt | B-T | High Level | MLB Years | Drafted From | # |
| 1. | Justin Upton | ss | ARI | 17 | 8/25/1987 | 6-3 | 205 | R-R | MLB | 2007-2007 | Great Bridge High School (Chesapeake,VA) | 4 |
| 2. | Alex Gordon | 3b | KC | 21 | 2/10/1984 | 6-1 | 220 | L-R | MLB | 2007-2007 | University of Nebraska | 117 |
| 3. | Jeff Clement | c | SEA | 21 | 8/21/1983 | 6-1 | 210 | L-R | MLB | 2007-2007 | USC | 270 |
| 4. | Ryan Zimmerman | 3b | WAN | 20 | 9/28/1984 | 6-2 | 210 | R-R | MLB | 2005-2007 | University of Virginia | 56 |
| 5. | Ryan Braun | 3b | MIL | 21 | 11/17/1983 | 6-2 | 205 | R-R | MLB | 2007-2007 | University of Miami (Florida) | 191 |
| 6. | Ricky Romero | lhp | TOR | 20 | 11/6/1984 | 6-1 | 195 | R-L | AA | | California State University, Fullerton | 211 |
| 7. | Troy Tulowitzki | ss | COL | 20 | 10/10/1984 | 6-3 | 205 | R-R | MLB | 2006-2007 | Long Beach State University | 142 |
| 8. | Wade Townsend | rhp | TB | 22 | 2/22/1983 | 6-4 | 230 | R-R | A | | Dripping Springs High School (Dripping Springs,TX) | 2 |
| 9. | Mike Pelfrey | rhp | NYM | 21 | 1/14/1984 | 6-7 | 190 | R-R | MLB | 2006-2007 | Wichita State University | 127 |
| 10. | Cameron Maybin | of | DET | 18 | 4/4/1987 | 6-3 | 200 | R-R | MLB | 2007-2007 | T.C. Roberson High School (Asheville,NC) | 7 |
| 11. | Andrew McCutchen | of | PIT | 18 | 10/10/1986 | 5-11 | 175 | R-R | AAA | | Fort Meade High School (Fort Meade,FL) | 4 |
| 12. | Jay Bruce | of | CIN | 18 | 4/3/1987 | 6-3 | 195 | L-L | AAA | | West Brook High School (Beaumont,TX) | 1 |
| 13. | Brandon Snyder | c | BAL | 18 | 11/23/1986 | 6-2 | 205 | R-R | A | | Westfield High School (Centreville,VA) | 1 |
| 14. | Trevor Crowe | of | CLE | 21 | 11/17/1983 | 6-0 | 190 | B-R | AA | | University of Arizona | 180 |
| 15. | Lance Broadway | rhp | CHW | 21 | 8/20/1983 | 6-4 | 190 | R-R | MLB | 2007-2007 | Texas Christian University | 74 |
| 16. | Chris Volstad | rhp | FLA | 18 | 9/23/1986 | 6-8 | 220 | R-R | AA | | Palm Beach Gardens High School (Palm Beach Gardens,FL) | 8 |
| 17. | C.J. Henry | ss | NYY | 19 | 5/31/1986 | 6-3 | 205 | R-R | A | | Putnam City High School (Oklahoma City,OK) | 6 |
| 18. | Cesar Carrillo | rhp | SD | 21 | 4/29/1984 | 6-3 | 177 | R-R | AAA | | University of Miami (Florida) | 192 |
| 19. | John Mayberry | of | TEX | 21 | 12/21/1983 | 6-5 | 230 | R-R | AA | | Stanford University | 214 |
| 20. | Mark Pawelek | lhp | CHC | 18 | 8/18/1986 | 6-3 | 190 | L-L | A | | Springville High School (Springville,UT) | 4 |
| 21. | Cliff Pennington | ss | OAK | 21 | 6/15/1984 | 5-11 | 175 | B-R | AA | | Texas A&M University | 165 |
| 22. | Aaron Thompson | lhp | FLA | 18 | 2/28/1987 | 6-3 | 195 | L-L | A+ | | Second Baptist School High School (Houston,TX) | 2 |
| 23. | Jacoby Ellsbury | of | BOS | 21 | 9/11/1983 | 6-1 | 185 | L-L | MLB | 2007-2007 | Oregon State University | 81 |
| 24. | Brian Bogusevic | lhp | HOU | 21 | 2/18/1984 | 6-3 | 215 | L-L | AA | | Tulane University | 74 |
| 25. | Matt Garza | rhp | MIN | 21 | 11/11/1983 | 6-4 | 205 | R-R | MLB | 2006-2007 | Fresno State University | 166 |
| 26. | Craig Hansen | rhp | BOS | 21 | 11/15/1983 | 6-6 | 210 | R-R | MLB | 2005-2006 | St. John's University | 80 |
| 27. | Joey Devine | rhp | ATL | 21 | 9/19/1983 | 6-0 | 210 | R-R | MLB | 2005-2007 | North Carolina State University | 89 |
| 28. | Colby Rasmus | of | STL | 18 | 8/11/1986 | 6-1 | 175 | L-L | AA | | Russell County High School (Victorville,CA) | 1 |
| 29. | Jacob Marceaux | rhp | FLA | 21 | 2/14/1984 | 6-1 | 195 | R-R | A+ | | McNeese State University | 36 |
| 30. | Tyler Greene | ss | STL | 21 | 8/17/1983 | 6-2 | 185 | R-R | AA | | Georgia Tech | 121 |
| 31. | Matt Torra | rhp | ARI | 21 | 6/29/1984 | 6-3 | 225 | R-R | A+ | | University of Massachusetts | 55 |
| 32. | Chaz Roe | rhp | COL | 18 | 10/9/1986 | 6-5 | 180 | R-R | A+ | | Lafayette High School (Lexington,KY) | 9 |
| 33. | John Drennen | of | CLE | 18 | 8/26/1986 | 5-11 | 185 | L-L | A+ | | Rancho Bernardo High School (San Diego,CA) | 16 |
| 34. | Ryan Tucker | rhp | FLA | 18 | 12/6/1986 | 6-2 | 190 | R-R | A+ | | Temple City High School (Temple City,CA) | 5 |
| 35. | Cesar Ramos | lhp | SD | 21 | 6/22/1984 | 6-2 | 190 | L-L | AA | | Long Beach State University | 143 |
| 36. | Travis Buck | of | OAK | 21 | 11/18/1983 | 6-3 | 210 | L-L | MLB | 2007-2007 | Arizona State University | 341 |
| 37. | Trevor Bell | rhp | LAA | 18 | 10/12/1986 | 6-1 | 190 | L-R | A | | Crescenta Valley High School (La Crescenta,CA) | 16 |
| 38. | Eli Iorg | of | HOU | 22 | 3/14/1983 | 6-3 | 200 | R-R | A+ | | University of Tennessee | 103 |
| 39. | Henry Sanchez | 1b | MIN | 18 | 11/29/1986 | 6-3 | 235 | R-R | A | | Mission Bay High School (San Diego,CA) | 23 |
| 40. | Luke Hochevar | rhp | LA | 21 | 9/15/1983 | 6-5 | 205 | R-R | MLB | 2007-2007 | University of Tennessee | 104 |
| 41. | Beau Jones | lhp | ATL | 18 | 8/25/1986 | 6-1 | 195 | L-L | A+ | | Destrehan High School (Destrehan,LA) | 1 |
| 42. | Clay Buchholz | rhp | BOS | 20 | 8/14/1984 | 6-3 | 190 | L-R | MLB | 2007-2007 | Angelina College | 48 |
| 43. | Mark Mccormick | rhp | STL | 21 | 10/15/1983 | 6-2 | 195 | R-R | A+ | | Baylor University | 88 |
| 44. | Sean West | lhp | FLA | 19 | 6/15/1986 | 6-8 | 200 | L-L | A | | Captain Shreve High School (Shreveport,LA) | 4 |
| 45. | Jed Lowrie | 2b | BOS | 21 | 4/17/1984 | 6-0 | 180 | B-R | AAA | | Stanford University | 215 |
| 46. | Tyler Herron | rhp | STL | 18 | 8/5/1986 | 6-3 | 190 | R-R | A | | Wellington High School (Wellington,FL) | 15 |
| 47. | Michael Bowden | rhp | BOS | 18 | 9/9/1986 | 6-3 | 215 | R-R | AA | | Waubonsie Valley High School (Aurora,IL) | 3 |
| 48. | Garrett Olson | lhp | BAL | 21 | 10/18/1983 | 6-1 | 200 | R-L | MLB | 2007-2007 | Cal Poly | 70 |
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