| 1 | 4 | June-Reg | Ryan Zimmerman | 3b | 20 | 6-2 | 210 | R | R | MLB | 2005-2009 | University of Virginia | $2,975,000 |
| 4 | 114 | June-Reg | Justin Maxwell | of | 21 | 6-5 | 225 | R | R | MLB | 2007-2009 | University of Maryland | $386,000 |
| 5 | 144 | June-Reg | Ryan Delaughter | of | 18 | 6-3 | 210 | R | R | A- | | Ryan (Denton,TX) | $187,500 |
| 6 | 174 | June-Reg | Marco Estrada | P | 21 | 6-0 | 180 | R | R | MLB | 2008-2009 | Long Beach State University | $152,000 |
| 7 | 204 | June-Reg | Mike Daniel | OF | 20 | 6-4 | 185 | L | R | AAA | | University of North Carolina | $115,000 |
| 8 | 234 | June-Reg | Jack Spradlin | P | 20 | 6-2 | 170 | R | L | AAA | | USC | $90,000 |
| 9 | 264 | June-Reg | John Howell | of | 22 | 6-2 | 205 | L | R | A | | University of Central Florida | $70,000 |
| 10 | 294 | June-Reg | Dee Brown | OF | 22 | 6-0 | 230 | R | R | AA | | University of Central Florida | $15,000 |
| 11 | 324 | June-Reg | John Lannan | P | 20 | 6-5 | 200 | L | L | MLB | 2007-2009 | Siena College | |
| 12 | 354 | June-Reg | Craig Stammen | P | 21 | 6-3 | 200 | R | R | MLB | 2009-2009 | University of Dayton | |
| 13 | 384 | June-Reg | Andre Enriquez | P | 21 | 6-3 | 212 | R | R | A- | | Le Moyne College | |
| 14 | 414 | June-Reg | Deryck Johnson | OF | 18 | 6-0 | 175 | L | L | Rookie | | Cypress Creek (Orlando,FL) | |
| 15 | 444 | June-Reg | Michael Wadkins | P | 17 | 6-1 | 187 | R | R | - | | Tolman (Pawtucket,RI) | |
| 16 | 474 | June-Reg | Josh Palm | P | 22 | 6-4 | 210 | R | R | A- | | Penn State University | |
| 17 | 504 | June-Reg | Eduardo Pichardo | P | 17 | 6-0 | 175 | R | R | Rookie | | Southridge (Miami,FL) | |
| 18 | 534 | June-Reg | Tim Pahuta | 1b | 22 | 6-4 | 225 | L | R | A+ | | Seton Hall University | |
| 19 | 564 | June-Reg | Bradley Clark | P | 18 | 6-6 | 200 | R | R | Rookie | | Sickles (Tampa,FL) | |
| 20 | 594 | June-Reg | Ricky Shefka | P | 21 | 6-2 | 200 | B | R | A | | Coastal Carolina University | |
| 21 | 624 | June-Reg | Coby Mavroulis | P | 22 | 6-3 | 180 | L | L | A | | Texas A&M University | |
| 22 | 654 | June-Reg | Antonio Evangelista | P | 23 | 6-4 | 215 | R | R | Ind | | Seminole State College | |
| 23 | 684** | June-Reg | Brett Jensen | P | 21 | 6-7 | 180 | R | R | AA | | University of Nebraska | |
| 24 | 714 | June-Reg | Jeffrey Taylor | P | 21 | 6-9 | 245 | R | R | A- | | Missouri Southern State University | |
| 25 | 744** | June-Reg | Josue Peley | ss | 17 | 6-0 | 177 | R | R | A | | Edouard Montpetit (Montreal,QC) | |
| 26 | 774 | June-Reg | Doug Thennis | 3B | 19 | 6-3 | 210 | R | R | Rookie | | American River College | |
| 27 | 804 | June-Reg | Andrew Lane | 2b | 20 | 6-0 | 185 | R | R | A+ | | Grand Canyon University | |
| 28 | 834 | June-Reg | Hunter Pace | of | 18 | 5-10 | 155 | R | R | Rookie | | Hamilton (Chandler,AZ) | |
| 29 | 864 | June-Reg | Pat Barnes | OF | 19 | 6-1 | 185 | L | L | - | | Southwest Mississippi Community College | |
| 30 | 894 | June-Reg | Brian Pruitt | 3b | 18 | 6-1 | 175 | R | R | A- | | Florida Christian (Miami,FL) | |
| 31 | 924** | June-Reg | Clayton Conner | 3b | 18 | 6-2 | 205 | R | R | A | | Pensacola Catholic (Pensacola,FL) | |
| 32 | 954 | June-Reg | Daniel Schuh | OF | 18 | 6-3 | 196 | R | R | College | | Bishop Kenny (Jacksonville,FL) | |
| 33 | 984 | June-Reg | Ryan Buchter | P | 18 | 6-3 | 200 | L | L | A | | Highland Regional (Blackwood,NJ) | |
| 34 | 1014 | June-Reg | Jordan Thibodeaux | P | 19 | 6-3 | 180 | L | L | College | | San Jacinto College | |
| 35 | 1044 | June-Reg | Matt Averitt | P | | | | | | - | | New Mexico Junior College | |
| 36 | 1074** | June-Reg | Brett Mcmillan | 1b | 21 | 6-5 | 225 | L | R | A+ | | UCLA | |
| 37 | 1104 | June-Reg | Brandon Hamilton | OF | 18 | 5-11 | 175 | L | L | - | | Woodlawn (Shreveport,LA) | |
| 38 | 1134 | June-Reg | Marcus Jones | of | 18 | 6-3 | 195 | R | R | A | | Landon School (Bethesda,MD) | |
| 39 | 1164** | June-Reg | Jake McCarter | P | 20 | 6-1 | 185 | R | R | A+ | | Navarro College | |
| 40 | 1193 | June-Reg | Anthony Williams | OF | 17 | 5-10 | 165 | R | R | - | | Mission Bay (San Diego,CA) | |
| 41 | 1222** | June-Reg | Tyler Moore | 1b | 18 | 6-1 | 209 | R | R | A | | Northwest Rankin (Brandon,MS) | |
| 42 | 1251 | June-Reg | P.J. Treadaway | P | | | | | | College | | Pearl River Community College | |
| 43 | 1280 | June-Reg | Scott Barnes | P | 17 | 6-3 | 175 | L | L | AA | | Cathedral (Springfield,MA) | |
| 44 | 1309 | June-Reg | Steven Hensley | P | 18 | 6-3 | 180 | R | R | AA | | Charles D. Owen (Black Mountain,NC) | |
| 45 | 1338 | June-Reg | Anthony Shawler | P | 18 | 6-3 | 192 | R | R | A | | Smith (Chesapeake,VA) | |
| 46 | 1367 | June-Reg | Ibrahim Lopez | OF | | | | | | - | | Lake City Community College | |
| 47 | 1396 | June-Reg | Luis Feliz | of | 19 | 6-1 | 190 | R | R | College | | New Brunswick (New Brunswick,NJ) | |
| 48 | 1424 | June-Reg | William Cherry | of | 18 | 6-1 | 195 | R | R | - | | Jenkins (Lakeland,FL) | |
| 49 | 1451 | June-Reg | Terrence Brown | P | 18 | 6-3 | 180 | R | R | - | | Bullard (Fresno,CA) | |
| 50 | 1478 | June-Reg | Robert Leonhardt | P | 20 | 6-5 | 185 | R | R | A | | Angelina College | |