| 1 | 11 | June-Reg | Neil Walker | C | 18 | 6-3 | 205 | B | R | MLB | 2009-2009 | Pine-Richland (Gibsonia,PA) | $1,950,000 |
| 2 | 52 | June-Reg | Brian Bixler | SS | 21 | 6-1 | 188 | R | R | MLB | 2008-2009 | Eastern Michigan University | $670,000 |
| 3 | 82 | June-Reg | Eddie Prasch | 3B | 18 | 6-1 | 180 | L | R | AA | | Milton (Alpharetta,GA) | $500,000 |
| 4 | 112 | June-Reg | Joe Bauserman | P | 18 | 6-2 | 220 | R | R | A | | Lincoln (Tallahassee,FL) | $300,000 |
| 5 | 142 | June-Reg | Kyle Bloom | P | 21 | 6-4 | 180 | R | L | AA | | Illinois State University | $200,000 |
| 6 | 172 | June-Reg | A.J. Johnson | OF | 20 | 6-1 | 190 | R | R | A | | Tallahassee Community College | $158,500 |
| 7 | 202 | June-Reg | Jason Quarles | P | 21 | 6-0 | 195 | R | R | A | | Southern University | $125,000 |
| 8 | 232 | June-Reg | Eric Ridener | P | 18 | 6-5 | 230 | R | R | Ind | | Taravella (Coral Springs,FL) | $100,000 |
| 9 | 262 | June-Reg | Christopher Covington | OF | 18 | 5-11 | 190 | R | R | Rookie | | Brookwood (Snellville,GA) | $75,000 |
| 10 | 292 | June-Reg | Derek Hankins | P | 20 | 6-4 | 190 | R | R | AA | | University of Memphis | $65,000 |
| 11 | 322 | June-Reg | Matt Guillory | P | 21 | 6-4 | 175 | R | R | A+ | | University of Louisiana-Monroe | |
| 12 | 352 | June-Reg | J.P. Padron | 3B | 18 | 6-1 | 220 | R | R | Rookie | | Clear Creek (League City,TX) | |
| 13 | 382 | June-Reg | Brett Grandstrand | 2B | 21 | 6-0 | 175 | R | R | A | | Coastal Carolina University | |
| 14 | 412 | June-Reg | Jermel Lomack | 2B | 21 | 5-8 | 160 | B | R | A | | Prairie View A&M University | |
| 15 | 442** | June-Reg | John Slone | C | 21 | 6-2 | 195 | R | R | College | | Miami University | |
| 16 | 472 | June-Reg | Ryan Herbort | P | 18 | 6-2 | 200 | R | R | Rookie | | Northwest Whitfield (Tunnel Hill,GA) | |
| 17 | 502 | June-Reg | Matt Bishop | P | 22 | 6-2 | 215 | R | R | A+ | | East Carolina University | |
| 18 | 532 | June-Reg | Cory Luebke | P | 19 | 6-4 | 200 | R | L | AA | | Marion Catholic (Marion,OH) | |
| 19 | 562 | June-Reg | Brad Clapp | P | 18 | 6-4 | 215 | R | R | A | | Juanita (Kirkland,WA) | |
| 20 | 592 | June-Reg | Brandon Reddinger | C | 21 | 6-2 | 210 | R | R | A | | Washington State University | |
| 21 | 622 | June-Reg | Derek Drage | P | 22 | 6-3 | 200 | R | R | A+ | | Missouri State University | |
| 22 | 652 | June-Reg | Mike Hofius | 1B | 22 | 6-2 | 210 | L | L | A | | Long Beach State University | |
| 23 | 682 | June-Reg | Dustin Craig | P | 22 | 6-4 | 240 | R | R | A+ | | Sam Houston State University | |
| 24 | 712 | June-Reg | Joe Salas | P | 22 | 5-10 | 200 | L | L | A- | | University of New Mexico | |
| 25 | 742 | June-Reg | Kevin Miller | P | 22 | 6-3 | 220 | R | R | A- | | Bucknell University | |
| 26 | 772 | June-Reg | Jason Tweedy | SS | 17 | 5-11 | 188 | L | R | A | | Newark Memorial (Newark,CA) | |
| 27 | 802 | June-Reg | Scott Loeffler | C | | | | | | - | | Lake City Community College | |
| 28 | 832 | June-Reg | Pat McAnaney | P | 18 | 6-3 | 200 | R | L | A+ | | Westhill (Syracuse,NY) | |
| 29 | 862 | June-Reg | Christian Romple | C | 19 | 6-3 | 195 | R | R | - | | Big Bend Community College | |
| 30 | 892 | June-Reg | Issael Gonzalez | 2B | 17 | 5-11 | 175 | R | R | Ind | | Edouard Montpetit (Quebec,QC) | |
| 31 | 922 | June-Reg | Oliver Marmol | SS | 17 | 5-10 | 165 | R | R | A+ | | Dr. Phillips (Orlando,FL) | |
| 32 | 952 | June-Reg | Chris Palma | P | | | | | | - | | College of the Canyons | |
| 33 | 982** | June-Reg | Justin Byler | C | 18 | 6-2 | 212 | R | R | A- | | Warren G. Harding (Warren,OH) | |
| 34 | 1012** | June-Reg | Nick Beghtol | C | | | | | | - | | Mountain View (Loveland,CO) | |
| 35 | 1042 | June-Reg | Jeff Robinson | P | | | | | | - | | Roswell (Roswell,GA) | |
| 36 | 1072 | June-Reg | Jose Nunez | SS | | | | | | - | | Puerto Rico Baseball Academy (Gurabo,PR) | |
| 37 | 1102 | June-Reg | Tim Rice | P | 20 | 6-2 | 200 | L | L | Rookie | | University of Richmond | |
| 38 | 1132 | June-Reg | Keith Pedersen | P | | | | | | - | | Glide (Glide,OR) | |
| 39 | 1162 | June-Reg | Todd Redmond | P | 19 | 6-3 | 185 | R | R | AAA | | St. Petersburg College | |
| 40 | 1192 | June-Reg | Jeremy Horst | P | 18 | 6-4 | 225 | L | L | AA | | Iowa Western Community College | |
| 41 | 1222 | June-Reg | Dan Schwartzbauer | SS | 22 | 5-11 | 175 | R | R | A+ | | Duquesne University | |
| 42 | 1251** | June-Reg | Mikel McIntyre | OF | 19 | 6-0 | 160 | R | R | - | | Diablo Valley College | |
| 43 | 1280 | June-Reg | Stephen Ashcraft | OF | 18 | 6-1 | 165 | L | L | - | | Brentwood Academy (Brentwood,TN) | |
| 44 | 1309 | June-Reg | Sean Setzer | 1B | | | | | | - | | Wilson (Portland,OR) | |
| 45 | 1338** | June-Reg | Corey Kemp | C | 18 | 6-0 | 240 | R | R | AA | | Centennial (Franklin,TN) | $0 |
| 46 | 1367 | June-Reg | Josh Scofield | P | 18 | 6-1 | 191 | L | L | College | | Newport (Bellevue,WA) | |
| 47 | 1396 | June-Reg | Zach Phillips | P | | | | | | - | | Lee County (Sanford,NC) | |
| 48 | 1425 | June-Reg | Rick Rivas | P | 20 | 6-1 | 180 | R | R | Ind | | El Paso Community College | |
| 49 | 1453 | June-Reg | Patrick Wandtke | P | | | | | | - | | West Orange (Winter Garden,FL) | |
| 50 | 1481 | June-Reg | Brock Lindeke | C | 17 | | | | | - | | Soquel (Soquel,CA) | |
