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| 1. | Bryan Bullington | | P | Pittsburgh Pirates | 21 | 9/30/1980 | 6-5 | 220 | R | R | MLB | 2005-2007 | Ball State University | 33 |
| 2. | B.J. Upton | | SS | Tampa Bay Devil Rays | 17 | 8/21/1984 | 6-2 | 170 | R | R | MLB | 2004-2007 | Greenbrier Christian Academy High School (Chesapeake,VA) | 2 |
| 3. | Chris Gruler | | P | Cincinnati Reds | 18 | 9/11/1983 | 6-3 | 210 | R | R | A | | Liberty Union High School (Brentwood,CA) | 2 |
| 4. | Adam Loewen | | P | Baltimore Orioles | 18 | 4/9/1984 | 6-6 | 220 | L | L | MLB | 2006-2007 | Fraser Valley Christian High School (Surrey,BC) | 1 |
| 5. | Clint Everts | | P | Montreal Expos | 17 | 8/10/1984 | 6-2 | 170 | R | R | A+ | | exists High School (-) | 67 |
| 6. | Zack Greinke | | P | Kansas City Royals | 18 | 10/21/1983 | 6-2 | 190 | R | R | MLB | 2004-2007 | Apopka High School (Apopka,FL) | 13 |
| 7. | Prince Fielder | | 1B | Milwaukee Brewers | 18 | 5/9/1984 | 5-11 | 280 | L | R | MLB | 2005-2007 | Eau Gallie High School (Melbourne,FL) | 9 |
| 8. | Scott Moore | | SS | Detroit Tigers | 18 | 11/17/1983 | 6-2 | 180 | L | R | MLB | 2006-2007 | Cypress High School (Cypress,CA) | 5 |
| 9. | Jeff Francis | | P | Colorado Rockies | 21 | 1/8/1981 | 6-5 | 200 | L | L | MLB | 2004-2007 | University of Lethbridge | 2 |
| 10. | Drew Meyer | | SS | Texas Rangers | 20 | 8/29/1981 | 5-10 | 180 | L | R | MLB | 2006-2006 | University of South Carolina | 98 |
| 11. | Jeremy Hermida | | OF | Florida Marlins | 18 | 1/30/1984 | 6-4 | 200 | L | R | MLB | 2005-2007 | exists High School (-) | 68 |
| 12. | Joe Saunders | | P | Anaheim Angels | 21 | 6/16/1981 | 6-3 | 195 | L | L | MLB | 2005-2007 | Virginia Tech | 56 |
| 13. | Khalil Greene | | SS | San Diego Padres | 22 | 10/21/1979 | 5-11 | 210 | R | R | MLB | 2003-2007 | Clemson University | 142 |
| 14. | Russ Adams | | SS | Toronto Blue Jays | 21 | 8/30/1980 | 6-1 | 178 | L | R | MLB | 2004-2007 | University of North Carolina | 117 |
| 15. | Scott Kazmir | | P | New York Mets | 18 | 1/24/1984 | 6-1 | 170 | L | L | MLB | 2004-2007 | exists High School (-) | 69 |
| 16. | Nick Swisher | | OF | Oakland Athletics | 21 | 11/25/1980 | 6-0 | 200 | B | L | MLB | 2004-2007 | Ohio State University | 81 |
| 17. | Cole Hamels | | P | Philadelphia Phillies | 18 | 12/27/1983 | 6-3 | 170 | L | L | MLB | 2006-2007 | Rancho Bernardo High School (San Diego,CA) | 12 |
| 18. | Royce Ring | | P | Chicago White Sox | 21 | 12/21/1980 | 6-5 | 220 | L | L | MLB | 2005-2007 | San Diego State University | 144 |
| 19. | James Loney | | 1B | Los Angeles Dodgers | 18 | 5/7/1984 | 6-3 | 200 | L | L | MLB | 2006-2007 | Elkins High School (Missouri City,TX) | 4 |
| 20. | Denard Span | | OF | Minnesota Twins | 18 | 2/27/1984 | 6-1 | 180 | L | L | AAA | | Tampa Catholic High School (Tampa,FL) | 13 |
| 21. | Bobby Brownlie | | P | Chicago Cubs | 21 | 10/5/1980 | 6-0 | 210 | R | R | AAA | | Rutgers University | 50 |
| 22. | Jeremy Guthrie | | P | Cleveland Indians | 23 | 4/8/1979 | 6-1 | 200 | B | R | MLB | 2004-2007 | Stanford University | 191 |
| 23. | Jeff Francoeur | | OF | Atlanta Braves | 18 | 1/8/1984 | 6-4 | 200 | R | R | MLB | 2005-2007 | Parkview High School (Liburn,GA) | 8 |
| 24. | Joe Blanton | | P | Oakland Athletics | 21 | 12/11/1980 | 6-2 | 220 | R | R | MLB | 2004-2007 | University of Kentucky | 75 |
| 25. | Matt Cain | | P | San Francisco Giants | 17 | 10/1/1984 | 6-3 | 180 | R | R | MLB | 2005-2007 | Houston High School (Germantown,TN) | 1 |
| 26. | John McCurdy | | IF | Oakland Athletics | 21 | 4/17/1981 | 6-2 | 200 | R | R | AA | | University of Maryland | 61 |
| 27. | Sergio Santos | | SS | Arizona Diamondbacks | 18 | 7/4/1983 | 6-3 | 190 | R | R | AAA | | Mater Dei High School (Santa Ana,CA) | 26 |
| 28.** | John Mayberry | | H | Seattle Mariners | 18 | 12/21/1983 | 6-5 | 230 | R | R | AA | | Rockhurst High School (Kansas City,MO) | 7 |
| 29. | Derick Grigsby | | P | Houston Astros | 20 | 6/30/1982 | 6-0 | 190 | R | R | A | | Northeast Texas Community College | 23 |
| 30. | Ben Fritz | | P | Oakland Athletics | 21 | 3/29/1981 | 6-4 | 225 | R | R | AAA | | Fresno State University | 155 |
| 31. | Greg Miller | | P | Los Angeles Dodgers | 17 | 11/3/1984 | 6-5 | 190 | L | L | AAA | | Esperanza High School (Anaheim,CA) | 18 |
| 32. | Luke Hagerty | | P | Chicago Cubs | 21 | 4/1/1981 | 6-7 | 230 | R | L | A+ | | Ball State University | 34 |
| 33. | Matt Whitney | | 3B | Cleveland Indians | 18 | 2/13/1984 | 6-4 | 190 | R | R | A+ | | Palm Beach Gardens High School (Palm Beach Gardens,FL) | 5 |
| 34. | Dan Meyer | | P | Atlanta Braves | 20 | 7/3/1981 | 6-3 | 190 | R | L | MLB | 2004-2007 | James Madison University | 57 |
| 35. | Jeremy Brown | | C | Oakland Athletics | 22 | 10/25/1979 | 5-10 | 210 | R | R | MLB | 2006-2006 | University of Alabama | 91 |
| 36. | Chadd Blasko | | P | Chicago Cubs | 21 | 3/9/1981 | 6-7 | 220 | R | R | AA | | Purdue University | 38 |
| 37. | Steve Obenchain | | P | Oakland Athletics | 20 | 7/29/1981 | 6-5 | 220 | R | R | AA | | University of Evansville | 25 |
| 38. | Matt Clanton | | P | Chicago Cubs | 21 | 4/16/1981 | 6-3 | 220 | B | R | A- | | Orange County Community College | 113 |
| 39. | Mark Teahen | | 3B | Oakland Athletics | 20 | 9/6/1981 | 6-3 | 210 | L | R | MLB | 2005-2007 | St. Mary's Collegiate | 1 |
| 40. | Mark Schramek | | P | Cincinnati Reds | 22 | 6/2/1980 | 6-3 | 230 | L | R | AA | | University of Texas | 229 |
| 41. | Micah Schilling | | 2B | Cleveland Indians | 19 | 12/27/1982 | 5-11 | 180 | L | R | A+ | | Sillman Institute | 1 |
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