| 1 | 2 | June-Reg | B.J. Upton | SS | 17 | 6-2 | 170 | R | R | MLB | 2004-2009 | Greenbrier Christian Academy (Chesapeake,VA) | $4,600,000 |
| 2 | 43 | June-Reg | Jason Pridie | OF | 18 | 6-1 | 195 | L | R | MLB | 2008-2009 | Prescott (Prescott,AZ) | $892,500 |
| 3 | 74 | June-Reg | Elijah Dukes | CF | 18 | 6-2 | 220 | R | R | MLB | 2007-2009 | Hillsborough (Tampa,FL) | $500,000 |
| 4 | 104 | June-Reg | Wes Bankston | OF | 18 | 6-4 | 200 | R | R | MLB | 2008-2008 | Plano East (Plano,TX) | |
| 5 | 134** | June-Reg | Mark Romanczuk | P | 18 | 6-1 | 190 | L | L | A | | St. Mark's (Wilmington,DE) | |
| 6 | 164** | June-Reg | Cesar Ramos | P | 18 | 6-2 | 190 | L | L | MLB | 2009-2009 | El Rancho (Pico Rivera,CA) | |
| 7 | 194 | June-Reg | Scott Autrey | P | 21 | 6-2 | 210 | R | R | AA | | University of North Carolina | |
| 8 | 224 | June-Reg | Joey Gomes | OF | 22 | 6-2 | 210 | R | R | AA | 2003-2004 | Santa Clara University | |
| 9 | 254** | June-Reg | Chris Leroux | C | 18 | 6-6 | 210 | L | R | MLB | 2009-2009 | St. Joseph (Mississauga,ON) | |
| 10 | 284 | June-Reg | Jason Hammel | P | 19 | 6-6 | 200 | R | R | MLB | 2006-2009 | Treasure Valley Community College | |
| 11 | 314 | June-Reg | Adam Moreno | P | 18 | 6-1 | 180 | R | R | Rookie | | Fresno (Fresno,CA) | |
| 12 | 344 | June-Reg | Blair Irvin | OF | 19 | 6-2 | 175 | L | R | Rookie | | Patterson (Patterson,LA) | |
| 13 | 374** | June-Reg | Matt Harrington | P | 20 | 6-4 | 210 | R | R | Ind | | | |
| 14 | 404 | June-Reg | Nick DeBarr | P | 18 | 6-4 | 220 | R | R | AAA | | Lassen Community College | |
| 15 | 434** | June-Reg | Mike Pelfrey | P | 18 | 6-7 | 190 | R | R | MLB | 2006-2009 | Wichita Heights (Wichita,KS) | |
| 16 | 464 | June-Reg | Michael Prochaska | P | 22 | 6-1 | 200 | L | L | AAA | | North Carolina State University | |
| 17 | 494 | June-Reg | Bud Woodruff | C | 19 | 6-4 | 200 | R | R | A- | | Gulf Coast Community College | |
| 18 | 524 | June-Reg | Romelio Lopez | P | 18 | 6-7 | 230 | B | R | A | | Conroe (Ciudad Bolivar,Ven) | |
| 19 | 554 | June-Reg | Cole Smith | P | 18 | 6-4 | 170 | R | R | Rookie | | Rockwall-Heath (Rockwall,TX) | |
| 20 | 584 | June-Reg | Jarrod Farrell | P | 18 | 6-6 | 180 | R | R | Rookie | | Hahnville (Boutte,LA) | |
| 21 | 614** | June-Reg | Chris Garcia | P | 17 | 6-1 | 205 | R | R | - | | Xaverian (Brooklyn,NY) | |
| 22 | 644 | June-Reg | Travis Ingle | | 20 | 6-3 | 200 | R | R | College | | Cal State Fullerton | |
| 23 | 674** | June-Reg | Jacoby Ellsbury | | 18 | 6-1 | 185 | L | L | MLB | 2007-2009 | Madras (Madras,OR) | |
| 24 | 704 | June-Reg | Adam Nikolic | OF | 20 | 5-9 | 190 | L | L | A | | Cal State Northridge | |
| 25 | 734 | June-Reg | Brian Bulger | P | 21 | 6-4 | 200 | R | R | A | | Georgia College & State University | |
| 26 | 764 | June-Reg | Jarrad Lavergne | P | 19 | 6-2 | 200 | L | L | A | | Westgate (New Iberia,LA) | |
| 27 | 794 | June-Reg | Brandon Mann | P | 18 | 6-2 | 160 | L | L | AA | | Mount Rainier (Des Moines,WA) | |
| 28 | 824 | June-Reg | Adam Olerio | P | 18 | 6-5 | 220 | R | R | - | | North Kingston (North Kingston,RI) | |
| 29 | 854 | June-Reg | Mike McGowan | P | | | 0 | | | - | | Navarro College | |
| 30 | 884 | June-Reg | Michael Geddes | P | 25 | 6-0 | 170 | R | R | Ind | | Jenison (Jenison,MI) | |
| 31 | 914 | June-Reg | Isiah Garner | P | 22 | 6-6 | 210 | L | L | Ind | | University of Arkansas-Pine Bluff | |
| 32 | 944 | June-Reg | Rodney Keener | P | | | 0 | | | - | | Minden (Minden,LA) | |
| 33 | 974 | June-Reg | Coltyn Simmons | OF | 18 | 6-0 | 190 | R | R | A+ | | Durango (Las Vegas,NV) | |
| 34 | 1004 | June-Reg | John Emmons | CF | 20 | 6-1 | 205 | R | R | AA | | Wharton County Junior College | |
| 35 | 1034 | June-Reg | Luke Cosmos | P | 18 | | 0 | | | - | | McAteer (San Francisco,CA) | |
| 36 | 1064** | June-Reg | Jake Muyco | C | 17 | 6-1 | 170 | R | R | AA | | Richland (Richland,WA) | |
| 37 | 1094 | June-Reg | Shane Shelley | SS | 18 | 5-11 | 170 | B | R | Rookie | | Belle Chasse (Belle Chasse,LA) | |
| 38 | 1124 | June-Reg | Matt Cobb | | 18 | 6-3 | 180 | L | L | A- | | Shelton State Community College | |
| 39 | 1154** | June-Reg | Brandon Rousseve | SS | 17 | 6-1 | 175 | R | R | A | | Holy Cross (New Orleans,LA) | |
| 40 | 1184 | June-Reg | Justin Hopes | P | | | 0 | | | Ind | | Jeanerette (Jeanerette,LA) | |
| 41 | 1214** | June-Reg | Daron Roberts | C | 19 | 6-0 | 215 | R | R | AA | | Cosumnes River College | |
| 42 | 1244** | June-Reg | Brandon Federici | P | 17 | 200 | | L | L | - | | Riverview (Oakmont,PA) | |
| 43 | 1274 | June-Reg | Steven Skinner | SS | 21 | 5-9 | 180 | R | R | Rookie | | Santa Rosa Junior College | |
| 44 | 1302 | June-Reg | Rolando Quinonez | C | | | 0 | | | - | | | |
| 45 | 1330 | June-Reg | Justin Standridge | P | | | 0 | | | - | | Clay Chalkville (Pinson,AL) | |
| 46 | 1357 | June-Reg | Jino Gonzalez | P | 19 | 6-2 | 210 | L | L | AA | | College of Southern Nevada | |
| 47 | 1383 | June-Reg | Adam Dalby | C | | | 0 | | | - | | Daingerfield (Daingerfield,TX) | |
| 48 | 1409** | June-Reg | Jason Urquidez | P | 19 | 6-2 | 175 | R | R | AAA | | Central Arizona College | |
| 49 | 1435 | June-Reg | Michael Sanders | | | | | | | - | | University of Alabama | |
| 50 | 1459 | June-Reg | Bryan Banks | P | | | 0 | | | - | | St. Petersburg College | |