| 1 | 12 | June-Reg | Mike Jones | P | 18 | 6-4 | 210 | R | R | AAA | | Thunderbird (Phoenix,AZ) | $1,825,000 |
| 2 | 56 | June-Reg | J.J. Hardy | IF | 18 | 6-2 | 205 | R | R | MLB | 2005-2009 | Sabino (Tucson,AZ) | $740,000 |
| 3 | 88 | June-Reg | Jon Steitz | P | 20 | 6-3 | 210 | R | R | A | | Yale University | $825,000 |
| 4 | 118 | June-Reg | Brad Nelson | IF | 18 | 6-2 | 230 | L | R | MLB | 2008-2009 | | $400,000 |
| 5 | 148 | June-Reg | Judd Richardson | P | 21 | 6-4 | 200 | L | R | A | | Miami University | $185,000 |
| 6 | 178 | June-Reg | Calvin Carpenter | P | 18 | 6-2 | 190 | R | R | A | | Natchitoches Central (Natchitoches,LA) | $184,000 |
| 7 | 208 | June-Reg | Taylor McCormack | 3B | 18 | 6-2 | 190 | R | R | Ind | | | $175,000 |
| 8 | 238 | June-Reg | Brandon Gemoll | 1B-OF | 20 | 6-2 | 210 | L | L | AAA | | Fresno State University | $83,000 |
| 9 | 268 | June-Reg | Dennis Sarfate | P | 20 | 6-4 | 210 | R | R | MLB | 2006-2009 | Chandler-Gilbert Community College | $85,600 |
| 10 | 298 | June-Reg | Greg Moreira | P | 18 | 6-5 | 210 | R | R | A | | Lake Brantley (Altamonte Springs,FL) | $135,000 |
| 11 | 328 | June-Reg | David Slevin | 2B | 20 | 5-10 | 170 | R | R | A- | | Indian River (IN CC) | |
| 12 | 358** | June-Reg | Ray Liotta | P | 18 | 6-3 | 220 | L | L | AAA | | | |
| 13 | 388 | June-Reg | Travis Hinton | OF | 20 | 6-1 | 210 | L | L | A+ | | Chandler-Gilbert Community College | |
| 14 | 418 | June-Reg | Aaron Sheffield | P | 19 | 6-3 | 215 | R | R | Rookie | | Young Harris College | |
| 15 | 448** | June-Reg | Tim Dillard | C | 17 | 6-4 | 200 | B | R | MLB | 2008-2009 | Saltillo (Saltillo,MS) | |
| 16 | 478 | June-Reg | Gene Desalme | P | 21 | 6-5 | 210 | L | L | Ind | | Northwestern State University | |
| 17 | 508** | June-Reg | Justin Wilson | P | 19 | 6-2 | 195 | L | L | Rookie | | Chandler (Chandler,AZ) | |
| 18 | 538 | June-Reg | Jeffrey Eure | 1B | 20 | 6-1 | 200 | L | L | AA | | Old Dominion University | |
| 19 | 568 | June-Reg | Joel Alvarado | C | 21 | 6-2 | 190 | R | R | AA | | University of Texas | |
| 20 | 598** | June-Reg | Josh Smith | P | 17 | 6-4 | 240 | R | R | College | | Lake Havasu (Lake Havasu,AZ) | |
| 21 | 628 | June-Reg | Orlando Viera | H | 17 | 6-0 | 170 | L | L | Rookie | | Dra Conchita Cuevas (Gurabo,PR) | |
| 22 | 658 | June-Reg | Damarius Bilbo | OF | 18 | 6-3 | 215 | R | R | - | | Moss Point (Moss Point,MS) | |
| 23 | 688 | June-Reg | David Nolasco | P | 22 | 6-2 | 200 | R | R | AA | | Riverside Community College | |
| 24 | 718 | June-Reg | Dan Kolb | P | 21 | 6-1 | 190 | R | R | AAA | 2006-2006 | State College of Florida, Manatee-Sarasota | |
| 25 | 748 | June-Reg | Chris Barnwell | IF | 22 | 5-10 | 180 | R | R | MLB | 2006-2006 | Flagler College | |
| 26 | 778 | June-Reg | Manny Parra | P | 18 | 6-3 | 200 | L | L | MLB | 2007-2009 | American River College | $1,550,000 |
| 27 | 808 | June-Reg | Dan Boyd | OF | 22 | 6-0 | 185 | R | R | A+ | | University of South Florida | |
| 28 | 838 | June-Reg | Chris Saenz | P | 19 | 6-3 | 215 | R | R | MLB | 2004-2004 | Pima Community College | |
| 29 | 868 | June-Reg | Jamie McAlister | P | 18 | 6-2 | 195 | R | R | College | | Clearwater (Piedmont,MO) | |
| 30 | 898 | June-Reg | Chris Gittlings | P | 18 | 6-3 | 190 | R | R | - | | Desales (Louisville,KY) | |
| 31 | 928 | June-Reg | Andy Sigerich | | 18 | 6-9 | 240 | R | R | College | | Downers Grove North (Downers Grove,IL) | |
| 32 | 958 | June-Reg | Jon Calmes | P | 18 | 6-4 | 160 | R | R | - | | Eastlake (Sammamish,WA) | |
| 33 | 988 | June-Reg | Greg Esteves | SS | 18 | 6-1 | 180 | R | R | - | | River Ridge (New Port Richey,FL) | |
| 34 | 1018 | June-Reg | Fuarieuir Miller | CF | | | 0 | | | - | | Laney College | |
| 35 | 1048 | June-Reg | Stephen Hunt | OF | 19 | 6-3 | 190 | L | R | Rookie | | Chandler-Gilbert Community College | |
| 36 | 1078 | June-Reg | Rusty Huggins | P | 21 | 6-2 | 205 | L | L | Rookie | | Central Florida Community College | |
| 37 | 1108 | June-Reg | Tom Carrow | OF | 19 | 6-2 | 185 | R | R | A | | University of Tampa | |
| 38 | 1138** | June-Reg | Ross Hawley | P | 21 | 6-0 | 175 | R | R | A | | Augustana College | |
| 39 | 1168 | June-Reg | Travis Johnson | CF | | | 0 | | | - | | Shannon (Shannon,MS) | |
| 40 | 1198 | June-Reg | Jeremy Wilson | C | 18 | 6-2 | 205 | R | R | College | | Catholic Central (Burlington,WI) | |
| 41 | 1227 | June-Reg | Hubert Pruett | P | 18 | 6-2 | 220 | R | R | A+ | | Kamehameha (Honolulu,HI) | |
| 42 | 1256 | June-Reg | Ralph Santana | IF | 20 | 6-1 | 175 | L | R | A+ | | Lake Sumter Community College | |
| 43 | 1284 | June-Reg | Matt Serafini | C | 21 | 6-1 | 230 | R | R | A | | University of Evansville | |
| 44 | 1312 | June-Reg | Corry Parrott | CF | 21 | 6-1 | 190 | R | R | Rookie | | Long Beach State University | |
| 45 | 1340 | June-Reg | Jason Costello | P | 18 | 6-4 | 190 | R | L | College | | Clearwater (Clearwater,FL) | |
| 46 | 1368 | June-Reg | Jordan Szabo | | 17 | 6-3 | 195 | R | R | A | | | |
| 47 | 1395 | June-Reg | Joseph Costentino | RF | 18 | 5-11 | 175 | R | R | - | | | |
| 48 | 1422 | June-Reg | Brian Harper | C | | | | | | - | | North Little Rock (North Little Rock,AR) | |
| 49 | 1447 | June-Reg | Nicholas Stillwagon | C | 18 | 6-0 | 190 | R | R | A | | Biloxi (Biloxi,MS) | |
| 50 | 1470 | June-Reg | Chris Haggard | | 22 | 6-1 | 190 | R | R | Rookie | | University of Oklahoma | |