| 1 | 16 | June-Reg | Lance Berkman | 1B | 21 | 6-1 | 205 | B | L | MLB | 1999-2009 | Rice University | $1,000,000 |
| 2 | 68 | June-Reg | Camron Hahn | C | 18 | 6-2 | 200 | R | R | Rookie | | Male (Louisville,KY) | |
| 3 | 100 | June-Reg | Scott Barrett | P | 18 | 5-10 | 175 | L | L | A- | | Mayde Creek (Houston,TX) | |
| 4 | 130** | June-Reg | Eric Byrnes | OF | 21 | 6-2 | 205 | R | R | MLB | 2000-2009 | UCLA | |
| 5 | 160 | June-Reg | Derek Stanford | P | 18 | 6-2 | 190 | R | R | A | | Temple (Temple,TX) | |
| 6 | 190 | June-Reg | Joe Messman | P | 21 | 6-2 | 175 | R | R | AA | | Oregon State University | |
| 7 | 220 | June-Reg | Bobby Bystrowski | OF | 20 | 6-1 | 190 | R | R | A+ | | Sacramento City College | |
| 8 | 250 | June-Reg | Ryan Dunn | OF | 20 | 6-2 | 200 | L | R | A- | | Texas Christian University | |
| 9 | 280 | June-Reg | Don Thomas | P | 21 | 6-2 | 170 | L | L | A+ | | Kennesaw State University | |
| 10 | 310 | June-Reg | Scott Fredericks | P | | | | | | - | | Saguaro (Scottsdale,AZ) | |
| 11 | 340** | June-Reg | Javier Pamus | P | 22 | 6-1 | 195 | R | R | AA | | San Jose State University | |
| 12 | 370 | June-Reg | Peter Sullivan | p | 22 | 6-5 | 225 | R | R | A- | | Limestone College | |
| 13 | 400 | June-Reg | Bart Leahy | OF | 20 | 6-5 | 225 | R | R | A- | | Ohio University | |
| 14 | 430** | June-Reg | Trey Hodges | p | 19 | 6-3 | 187 | R | R | MLB | 2002-2003 | Blinn College | |
| 15 | 460 | June-Reg | J.J. Thomas | 1B | 21 | 6-4 | 215 | R | R | A+ | | Georgia Tech | |
| 16 | 490 | June-Reg | Jim Wallace | p | 21 | 6-4 | 195 | R | R | A+ | | University of North Carolina | |
| 17 | 520 | June-Reg | Pat Cutshall | SS | 22 | 5-9 | 175 | R | R | A | | Mercyhurst College | |
| 18 | 550 | June-Reg | Simon Mitchell | OF | | | | | | - | | West Seattle (Seattle,WA) | |
| 19 | 580 | June-Reg | Brian Hecht | p | 19 | 6-4 | 205 | R | R | A | | University of Illinois | |
| 20 | 610 | June-Reg | Tim Redding | p | 19 | 6-0 | 215 | R | R | MLB | 2001-2009 | Monroe Community College | |
| 21 | 640** | June-Reg | Neal Maybin | OF | | | | | | Rookie | | Lake Howell (Winter Park,FL) | $0 |
| 22 | 670 | June-Reg | Jason Alfaro | p | 19 | 5-10 | 189 | R | R | MLB | 2004-2004 | Hill College | |
| 23 | 700 | June-Reg | Kyle Logan | OF-3B | 21 | 6-0 | 202 | L | R | AAA | | University of Southern Mississippi | |
| 24 | 730 | June-Reg | Tim Judd | p | | | | | | - | | Dixon (Dixon,CA) | |
| 25 | 760** | June-Reg | Charlton Jimerson | OF | 17 | 6-3 | 205 | R | R | MLB | 2005-2008 | Mount Eden (Hayward,CA) | |
| 26 | 790 | June-Reg | Jared Wood | p | | | | | | - | | St. Louis Community College-Meramec | |
| 27 | 820** | June-Reg | Derek Brewster | p | 18 | 5-11 | 220 | R | R | A- | | | |
| 28 | 850 | June-Reg | Graham Travis | p | | | | | | - | | Ballard (Seattle,WA) | |
| 29 | 880 | June-Reg | Joe Cathey | SS | 21 | 5-9 | 175 | B | R | A | | Rice University | |
| 30 | 910 | June-Reg | Christian Ross | p | 18 | 6-3 | 200 | R | R | Rookie | | Ringgold (Ringgold,GA) | |
| 31 | 940** | June-Reg | Kris Clute | SS | 18 | 5-9 | 175 | R | R | AA | | Killian (Miami,FL) | |
| 32 | 970 | June-Reg | Eric Ambruster | 1B | | | | | | - | | Kalamazoo Central (Kalamazoo,MI) | |
| 33 | 1000** | June-Reg | Lamont Matthews | Of | 19 | 6-2 | 210 | L | L | AAA | | Kishwaukee College | |
| 34 | 1030** | June-Reg | John Skinner | p | 20 | 6-1 | 185 | R | R | AA | | Chabot College | |
| 35 | 1060 | June-Reg | Kevin Marzion | p | 18 | | | | | College | | Elk Grove (Elk Grove,CA) | |
| 36 | 1090 | June-Reg | Jason Dill | 1B | 18 | 6-1 | 200 | L | L | A+ | - | Charlotte (Punta Gorda,FL) | |
| 37 | 1120** | June-Reg | Jerymane Beasley | Of | | | 0 | | | - | | Olympic College | |
| 38 | 1150 | June-Reg | John Colon | p | | | | | | - | | Molokai (Hoolehua,HI) | |
| 39 | 1180 | June-Reg | Marcos Rios | C | | | | | | - | | Chabot College | |
| 40 | 1210 | June-Reg | Garreth Perry | C | | | | | | - | | Service (Anchorage,AK) | |
