| Darren O'Day | RP | 33.276 | Baltimore Orioles | Baltimore Orioles (MLB) | Activated from DL |
| Mike Mayers | P | 24.232 | St. Louis Cardinals | St. Louis Cardinals (MLB) | Contract purchased from minors |
| Yolmer Sanchez | 3B | 24.026 | Chicago White Sox | Chicago White Sox (MLB) | Recalled from Charlotte (AAA) |
| Buck Farmer | RP | 25.155 | Detroit Tigers | Detroit Tigers (MLB) | Recalled from Toledo (AAA) |
| Anthony Ranaudo | RP | 26.319 | Chicago White Sox | Chicago White Sox (MLB) | Recalled from Charlotte (AAA) |
| Chris Sale | SP | 27.117 | Chicago White Sox | Chicago White Sox (MLB) | Suspended 5 games by team for tearing up throwback uniforms |
| Jesse Hahn | SP | 26.360 | Oakland Athletics | Oakland Athletics (MLB) | Recalled from Nashville (AAA) |
| Joe Nathan | RP | 41.245 | Chicago Cubs | Chicago Cubs (MLB) | Activated from DL |
| Lucas Giolito | P | 22.011 | Washington Nationals | Syracuse (AAA) | Assigned to Syracuse (AAA) |
| Ryan Tepera | RP | 28.265 | Toronto Blue Jays | Toronto Blue Jays (MLB) | Recalled from Buffalo (AAA) |
| Drew Storen | RP | 28.349 | Toronto Blue Jays | Toronto Blue Jays (MLB) | Designated for Assignment |
| AJ Schugel | P | 27.028 | Pittsburgh Pirates | Pittsburgh Pirates (MLB) | Recalled from Indianapolis (AAA) |
| Tyler Glasnow | P | 22.336 | Pittsburgh Pirates | Pittsburgh Pirates (MLB) | DL-15 Right shoulder soreness |
| Gavin Floyd | RP | 33.179 | Toronto Blue Jays | Toronto Blue Jays (MLB) | Transferred to 60-day DL |
| Joe Kelly | SP | 28.046 | Boston Red Sox | Boston Red Sox (MLB) | Recalled from Pawtucket (AAA) |
| Daniel Alvarez | p | 20.027 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Harold Arauz | P | 21.057 | Philadelphia Phillies | Gulf Coast Phillies (Rk) | Assigned to Gulf Coast Phillies (Rk) |
| Keyder Aristigueta | 2b | 20.174 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Dakota Bacus | P | 25.114 | Washington Nationals | Auburn (A-) | Assigned to Auburn (A-) |
| Homer Bailey | SP | 30.083 | Cincinnati Reds | Louisville (AAA) | Assigned to Louisville (AAA) for rehab |
| Patrick Baker | P | 22.344 | Baltimore Orioles | Delmarva (A) | Activated from disabled list |
| Agapito Barrios | p | 22.237 | Houston Astros | Quad Cities (A) | Assigned to Quad Cities (A) |
| Moises Bello | 3b | 19.042 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Parker Berberet | C-1B | 26.278 | Milwaukee Brewers | Colorado Springs (AAA) | Assigned to Colorado Springs (AAA) |
| Lisandro Blanco | of | 19.193 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Aaron Bossi | c | 22.276 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Silvino Bracho | RP | 24.008 | Arizona Diamondbacks | Reno (AAA) | Assigned to Reno (AAA) |
| Havid Burgos | p | 21.353 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Oswaldo Cabrera | ss | 17.146 | New York Yankees | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Matt Carasiti | P | 25.002 | Colorado Rockies | Albuquerque (AAA) | Assigned to Albuquerque (AAA) |
| Jodd Carter | OF | 20.005 | Cleveland Indians | Lynchburg (A+) | Assigned to Lynchburg (A+) |
| Moises Cedeno | p | 20.331 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Trent Grisham | of | 19.266 | Milwaukee Brewers | Wisconsin (A) | Activated from disabled list |
| Yeison Corredera | ss | 22.176 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Isrrael De la Cruz | ss | 19.040 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Bairon De La Rosa | p | 20.008 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Wilmer Dominguez | c | 26.036 | Tampa Bay Rays | Gulf Coast Rays (Rk) | Activated from disabled list |
| Brayan Emery | of | 18.132 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Raynel Espinal | p | 22.292 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Buck Farmer | RP | 25.155 | Detroit Tigers | Toledo (AAA) | Assigned to Toledo (AAA) |
| Kyle Farmer | IF | 25.342 | Los Angeles Dodgers | Tulsa (AA) | Assigned to Tulsa (AA) |
| Daniel Fields | CF | 25.183 | Los Angeles Dodgers | Tulsa (AA) | Activated from disabled list |
| Alfredo Figaro | RP | 32.018 | Los Angeles Dodgers | Tulsa (AA) | Assigned to Tulsa (AA) |
| Wilmer Font | RP | 26.062 | Toronto Blue Jays | New Hampshire (AA) | Assigned to New Hampshire (AA) |
| Nolan Fontana | IF | 25.049 | Houston Astros | Fresno (AAA) | Assigned to Fresno (AAA) |
| Michael Freeman | p | 24.292 | Houston Astros | Corpus Christi (AA) | Activated from disabled list |
| Edison Frias | P | 25.219 | Houston Astros | Fresno (AAA) | Assigned to Fresno (AAA) |
| Jose Fuentes | p | 21.292 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Drew Gagnon | P | 26.029 | Milwaukee Brewers | Colorado Springs (AAA) | Activated from disabled list |
| Alexis Garcia | ss | 19.024 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Jairo Garcia | p | 21.181 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Joxelier Garcia | 3B | 22.086 | Chicago White Sox | Winston-Salem (A+) | Assigned to Winston-Salem (A+) |
| Jack Goihl | c | 23.145 | Cleveland Indians | Mahoning Valley (A-) | Activated from disabled list |
| Gerardo Gonzalez | ss | 17.216 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Jose Gonzalez | of | 18.011 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Victor Gonzalez | P | 20.252 | Los Angeles Dodgers | Great Lakes (A) | Activated from disabled list |
| Trevor Gott | RP | 23.333 | Washington Nationals | Gulf Coast Nationals (Rk) | Activated from disabled list |
| Jesus Graterol | ss | 19.105 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Carlos Guzman | if | 18.070 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Jesse Hahn | SP | 26.360 | Oakland Athletics | Nashville (AAA) | Assigned to Nashville (AAA) |
| Spencer Hermann | P | 22.353 | Seattle Mariners | Bakersfield (A+) | Assigned to Bakersfield (A+) |
| Jefri Hernandez | P | 25.089 | Texas Rangers | Frisco (AA) | Assigned to Frisco (AA) |
| Wei-Chieh Huang | p | 22.302 | Arizona Diamondbacks | Hillsboro (A-) | Assigned to Hillsboro (A-) |
| Xavier Javier | p | 18.166 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Mason Katz | P | 25.336 | St. Louis Cardinals | Springfield (AA) | Activated from disabled list |
| Jon Keller | P | 23.351 | Baltimore Orioles | Frederick (A+) | Assigned to Frederick (A+) |
| Clyde Kendrick | p | 22.246 | Texas Rangers | Arizona Rangers (Rk) | Assigned to Arizona Rangers (Rk) |
| Nick Kingham | P | 24.260 | Pittsburgh Pirates | Gulf Coast Pirates (Rk) | Assigned to Gulf Coast Pirates (Rk) |
| Deshorn Lake | P | 22.269 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Trevor Lane | p | 22.090 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Jaron Long | P | 24.332 | Washington Nationals | Harrisburg (AA) | Assigned to Harrisburg (AA) |
| Ronaldo Lopez | p | 18.199 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Edel Luaces | of | 22.072 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Stanley Martinez | p | 21.238 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Algeni Mateo | C | 20.359 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Jhonny Mateo | p | 21.340 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Erick Matta | c | 22.107 | Texas Rangers | Frisco (AA) | Assigned to Frisco (AA) |
| Mike Mayers | P | 24.232 | St. Louis Cardinals | Memphis (AAA) | Assigned to Memphis (AAA) |
| Bringnel Mendez | p | 22.175 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Erick Mendez | of | 20.109 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Conner Menez | p | 21.057 | San Francisco Giants | Salem-Keizer (A-) | Assigned to Salem-Keizer (A-) |
| Melky Mesa | rf | 29.175 | Toronto Blue Jays | New Hampshire (AA) | Activated from disabled list |
| Melvin Morel | p | 22.223 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Erling Moreno | p | 19.193 | Chicago Cubs | Eugene (A-) | Assigned to Eugene (A-) |
| Preston Morrison | p | 23.006 | Chicago Cubs | Myrtle Beach (A+) | Assigned to Myrtle Beach (A+) |
| Raul Navarro | SS | 24.171 | Los Angeles Angels | Arizona Angels (Rk) | Assigned to Arizona Angels (Rk) |
| Chris Nelson | 3b | 30.325 | Colorado Rockies | Albuquerque (AAA) | Activated from disabled list |
| Antonio Nunez | SS | 23.196 | Houston Astros | Corpus Christi (AA) | Assigned to Corpus Christi (AA) |
| Jensen Park | of | 23.227 | Colorado Rockies | Asheville (A) | Assigned to Asheville (A) |
| Nick Pasquale | P | 25.271 | Cleveland Indians | Akron (AA) | Assigned to Akron (AA) |
| Dorssys Paulino | SS | 21.246 | Cleveland Indians | Arizona Indians (Rk) | Assigned to Arizona Indians (Rk) |
| Jose Paulino | P | 21.107 | Chicago Cubs | South Bend (A) | Assigned to South Bend (A) |
| David Peralta | LF | 28.346 | Arizona Diamondbacks | Reno (AAA) | Assigned to Reno (AAA) for rehab |
| Wladimir Pinto | p | 18.163 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Zac Reininger | P | 23.178 | Detroit Tigers | Gulf Coast Tigers (East) (Rk) | Activated from disabled list |
| Will Roberts | P | 25.342 | Cleveland Indians | Akron (AA) | Activated from disabled list |
| Terrance Robertson | of | 19.249 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Alexander Rodriguez | of | 18.134 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Brayan Rodriguez | c | 19.090 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Hector Rodriguez | p | 19.233 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Jesus Rodriguez | p | 18.159 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Reynaldo Rodriguez | 1B | 30.168 | Minnesota Twins | Rochester (AAA) | Assigned to Rochester (AAA) |
| Yonauris Rodriguez | ss | 19.137 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Jose Salas | ss | 19.099 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Kilber Santana | p | 17.283 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Jordan Scott | of | 19.063 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Matt Sergey | p | 26.361 | Oakland Athletics | Stockton (A+) | Activated from disabled list |
| Dylan Silva | p | 22.210 | Seattle Mariners | Clinton (A) | Activated from disabled list |
| Hector Silvestre | P | 23.223 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Bryson Smith | CF | 27.220 | Cincinnati Reds | Louisville (AAA) | Activated from disabled list |
| Andrew Sohn | IF | 23.078 | St. Louis Cardinals | Palm Beach (A+) | Assigned to Palm Beach (A+) |
| John Timmins | p | 22.186 | San Francisco Giants | Salem-Keizer (A-) | Assigned to Salem-Keizer (A-) |
| Cristhian Tortosa | p | 17.268 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Pedro Urena | of | 21.054 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Jesmuel Valentin | SS | 22.074 | Philadelphia Phillies | Lehigh Valley (AAA) | Assigned to Lehigh Valley (AAA) |
| Javier Villarroel | p | 18.267 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) |
| Brandon Wagner | 3b | 20.336 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Jonah Wesely | P | 21.229 | Los Angeles Angels | Orem (Rk) | Activated from disabled list |
| Garrett Williams | p | 21.313 | San Francisco Giants | Salem-Keizer (A-) | Assigned to Salem-Keizer (A-) |
| Miguel Yajure | p | 18.085 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Bobby Korecky | P | 36.313 | Toronto Blue Jays | Buffalo (AAA) | DL-7 Injured |
| Jeremy Hefner | SP | 30.136 | St. Louis Cardinals | Memphis (AAA) | DL-7 Injured |
| Layne Somsen | P | 27.050 | Los Angeles Dodgers | Oklahoma City (AAA) | DL-7 Injured |
| Jeremias Pineda | CF | 25.251 | Miami Marlins | Jupiter (A+) | Suspended |
| Jorge Ortega | p | 23.035 | Milwaukee Brewers | Biloxi (AA) | DL-7 Injured |
| Andy Fermin | 3B | 26.363 | Toronto Blue Jays | Dunedin (A+) | DL-7 Injured |
| Esmerling De La Rosa | p | 25.071 | Miami Marlins | Jupiter (A+) | Suspended |
| Javier Betancourt | SS-3B | 21.078 | Milwaukee Brewers | Biloxi (AA) | DL-7 Injured |
| Nick Travieso | P | 22.175 | Cincinnati Reds | Pensacola (AA) | DL-7 Injured |
| Carlos Polanco | P | 22.157 | Colorado Rockies | Modesto (A+) | DL-7 Injured |
| Rehiner Cordova | 2B | 22.195 | Miami Marlins | Jupiter (A+) | DL-7 Injured |
| Chase Williams | p | 23.244 | Milwaukee Brewers | Wisconsin (A) | DL-7 Injured |
| Tyler Stephenson | c | 19.343 | Cincinnati Reds | Dayton (A) | DL-7 Injured |
| Dionis Paulino | of | 22.035 | New York Mets | Brooklyn (A-) | DL-60 Injured |
| Edwin German | p | 23.318 | New York Mets | Kingsport (Rk) | DL-60 Injured |
| Gabe Howell | ss | 18.135 | Atlanta Braves | Danville (Rk) | DL-60 Injured |
| Jasseel De la Cruz | p | 19.029 | Atlanta Braves | Gulf Coast Braves (Rk) | Assigned to GCL Braves (Rk) |
| Josh Winckowski | p | 18.027 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Signed out of draft (Florida HS) |
| Jose Polonia | 3b | 20.227 | New York Yankees | Gulf Coast Yankees (Rk) | Assigned to GCL Yankees East (Rk) |
| Nicholas Shumpert | 2b | 19.256 | Atlanta Braves | Gulf Coast Braves (Rk) | Signed out of draft (San Jacinto) |
| Shean Michel | of | 18.302 | Atlanta Braves | Gulf Coast Braves (Rk) | Assigned to GCL Braves (Rk) |
| Sidney Duprey Conde | p | 19.252 | San Francisco Giants | Arizona Giants (Rk) | Signed out of draft (Kaskaskia CC) |
| Tolly Filotei | of | 20.240 | Chicago Cubs | Arizona Cubs (Rk) | Signed out of draft (Faulkner CC) |
