| Corey Knebel | RP | 24.196 | Milwaukee Brewers | Milwaukee Brewers (MLB) | Activated from DL |
| Jarrett Grube | P | 34.216 | | | Released by Indians |
| Joba Chamberlain | RP | 30.259 | Cleveland Indians | Cleveland Indians (MLB) | Activated from DL |
| Wandy Rodriguez | SP | 37.142 | | | Released by Orioles |
| Alex Guerrero | 3B | 29.171 | | | Released by Dodgers |
| Jameson Taillon | P | 24.204 | Pittsburgh Pirates | Pittsburgh Pirates (MLB) | Recalled from Indianapolis (AAA) |
| Christian Colon | 2B-3B | 27.026 | Kansas City Royals | Kansas City Royals (MLB) | Recalled from Omaha (AAA) |
| Williams Perez | SP | 25.019 | Atlanta Braves | Atlanta Braves (MLB) | DL-15 Strained right rotator cuff |
| Kelly Johnson | 2B | 34.107 | New York Mets | New York Mets (MLB) | Traded from Braves with cash to Mets for Akeel Morris |
| Josh Zeid | RP | 29.077 | New York Mets | New York Mets (MLB) | Signed to Minor League Contract |
| Justin Morneau | 1B | 35.025 | Chicago White Sox | Chicago White Sox (MLB) | Signed to 1-year contract for 2016-2016 ($1m) |
| Justin Morneau | 1B | 35.025 | Chicago White Sox | Chicago White Sox (MLB) | DL-15 Left elbow primary flexor |
| Osvaldo Abreu | SS | 21.361 | Washington Nationals | Potomac (A+) | Reinstated to active roster |
| Trever Adams | 1B | 27.252 | Texas Rangers | Round Rock (AAA) | Reinstated to active roster |
| Arismendy Alcantara | SS | 24.224 | Oakland Athletics | Nashville (AAA) | Traded from Cubs to Athletics for Chris Coghlan |
| Gaby Almonte | P | 23.298 | New York Mets | Columbia (A) | Assigned to Columbia (A) |
| Matt Alvarez | P | 25.149 | Kansas City Royals | Northwest Arkansas (AA) | Assigned to Northwest Arkansas (AA) |
| Cody Anderson | SP | 25.268 | Cleveland Indians | Columbus (AAA) | Assigned to Columbus (AAA) |
| Armando Araiza | C | 22.355 | Tampa Bay Rays | Charlotte (A+) | Assigned to Charlotte (A+) |
| Alex Balog | p | 23.328 | Colorado Rockies | Modesto (A+) | Assigned to Modesto (A+) |
| Tanner Banks | P | 24.229 | Chicago White Sox | Winston-Salem (A+) | Assigned to Winston-Salem (A+) |
| Corey Black | P | 24.310 | Chicago Cubs | Iowa (AAA) | Assigned to Iowa (AAA) |
| Devyn Bolasky | OF | 23.136 | New York Yankees | Tampa (A+) | Assigned to Tampa (A+) |
| David Bote | SS | 23.063 | Chicago Cubs | Myrtle Beach (A+) | Assigned to Myrtle Beach (A+) |
| Gabriel Bracamonte | c | 21.025 | Houston Astros | Lancaster (A+) | Assigned to Lancaster (A+) |
| Brandon Brennan | P | 24.319 | Chicago White Sox | Birmingham (AA) | Assigned to Birmingham (AA) |
| Jeimer Candelario | 3b | 22.197 | Chicago Cubs | Iowa (AAA) | Assigned to Iowa (AAA) |
| Tyler Carpenter | P | 24.105 | Los Angeles Angels | Arkansas (AA) | Activated from disabled list |
| Tyler Cravy | RP | 26.331 | Milwaukee Brewers | Colorado Springs (AAA) | Activated from disabled list |
| Tony Cruz | C | 29.295 | Kansas City Royals | Omaha (AAA) | Assigned to Omaha (AAA) |
| Miguel Del Castillo | C | 24.239 | San Diego Padres | San Antonio (AA) | Activated from disabled list |
| Natanael Delgado | LF | 20.230 | Baltimore Orioles | Delmarva (A) | Activated from disabled list |
| Dillon Dobson | ss | 22.292 | San Francisco Giants | Augusta (A) | Activated from disabled list |
| Blake Drake | OF | 22.333 | St. Louis Cardinals | Springfield (AA) | Assigned to Springfield (AA) |
| Frank Duncan | P | 24.131 | Pittsburgh Pirates | Indianapolis (AAA) | Assigned to Indianapolis (AAA) |
| Andrew Ely | IF | 23.137 | Chicago Cubs | Myrtle Beach (A+) | Assigned to Myrtle Beach (A+) |
| James Farris | P | 24.066 | Chicago Cubs | Tennessee (AA) | Assigned to Tennessee (AA) |
| Sam Gaviglio | P | 26.018 | Seattle Mariners | Jackson (AA) | Assigned to Jackson (AA) |
| Daniel Gibson | P | 24.237 | Arizona Diamondbacks | Reno (AAA) | Assigned to Reno (AAA) |
| Cade Gotta | OF | 24.313 | Tampa Bay Rays | Montgomery (AA) | Assigned to Montgomery (AA) |
| Jarrett Grube | P | 34.216 | Seattle Mariners | Tacoma (AAA) | Signed to Minor League Contract |
| Robert Gsellman | P | 22.326 | New York Mets | Las Vegas (AAA) | Assigned to Las Vegas (AAA) |
| Austin Hedges | C | 23.295 | San Diego Padres | El Paso (AAA) | Activated from disabled list |
| Hector Hernandez | P | 25.109 | Arizona Diamondbacks | Mobile (AA) | Assigned to Mobile (AA) |
| Samuel Hiciano | OF | 22.135 | Philadelphia Phillies | Clearwater (A+) | Assigned to Clearwater (A+) |
| Jordan Hillyer | p | 22.260 | Miami Marlins | Greensboro (A) | Assigned to Greensboro (A) |
| Ben Holmes | P | 24.271 | Miami Marlins | Jupiter (A+) | Assigned to Jupiter (A+) |
| Sam Howard | P | 23.096 | Colorado Rockies | Hartford (AA) | Assigned to Hartford (AA) |
| Travis Ishikawa | LF-1B | 32.259 | San Francisco Giants | Sacramento (AAA) | Signed to Minor League Contract |
| Luke Jackson | RP | 24.290 | Texas Rangers | Round Rock (AAA) | Assigned to Round Rock (AAA) |
| AJ Kennedy | c | 22.137 | San Diego Padres | Fort Wayne (A) | Assigned to Fort Wayne (A) |
| Colin Kleven | P | 25.055 | Chicago White Sox | Winston-Salem (A+) | Activated from disabled list |
| Logan Landon | of | 23.112 | Los Angeles Dodgers | Great Lakes (A) | Activated from disabled list |
| Artie Lewicki | P | 24.062 | Detroit Tigers | Lakeland (A+) | Activated from disabled list |
| Daniel Lockhart | SS-2B | 23.217 | Chicago Cubs | Tennessee (AA) | Assigned to Tennessee (AA) |
| Greg Mahle | RP | 23.053 | Los Angeles Angels | Salt Lake (AAA) | Assigned to Salt Lake (AAA) |
| Kevin McGowan | P | 24.235 | New York Mets | Binghamton (AA) | Assigned to Binghamton (AA) |
| Miguel Mejia | p | 28.142 | Chicago Cubs | Iowa (AAA) | Assigned to Iowa (AAA) |
| Rando Moreno | | 24.003 | San Francisco Giants | Sacramento (AAA) | Assigned to Sacramento (AAA) |
| Taylor Murphy | 3B | 23.218 | Cleveland Indians | Lynchburg (A+) | Assigned to Lynchburg (A+) |
| Curtis Partch | RP | 29.116 | Pittsburgh Pirates | Indianapolis (AAA) | Assigned to Indianapolis (AAA) |
| David Peterson | P | 26.156 | Atlanta Braves | Mississippi (AA) | Assigned to Mississippi (AA) |
| Arturo Rodriguez | C | 24.250 | Miami Marlins | Greensboro (A) | Assigned to Greensboro (A) |
| Luigi Rodriguez | CF | 23.208 | Cleveland Indians | Akron (AA) | Reinstated to active roster |
| Richard Rodriguez | 3b | 23.249 | San Francisco Giants | San Jose (A+) | Assigned to San Jose (A+) |
| Avery Romero | SS | 23.029 | Miami Marlins | Jupiter (A+) | Activated from disabled list |
| Nick Sarianides | P | 26.284 | Chicago Cubs | Tennessee (AA) | Assigned to Tennessee (AA) |
| Kyle Skipworth | C | 26.100 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) for rehab |
| Domingo Tapia | P | 24.176 | New York Mets | St. Lucie (A+) | Activated from disabled list |
| Dylan Tice | 2b | 23.176 | St. Louis Cardinals | Palm Beach (A+) | Assigned to Palm Beach (A+) |
| Damek Tomscha | 3B | 24.287 | Philadelphia Phillies | Lakewood (A) | Activated from disabled list |
| Jen-Ho Tseng | P | 21.249 | Chicago Cubs | Tennessee (AA) | Activated from disabled list |
| Jose Valdez | P | 26.100 | Los Angeles Angels | Salt Lake (AAA) | Assigned to Salt Lake (AAA) |
| Phillips Valdez | P | 24.206 | Washington Nationals | Harrisburg (AA) | Assigned to Harrisburg (AA) |
| Breyvic Valera | 2B | 23.312 | St. Louis Cardinals | Memphis (AAA) | Assigned to Memphis (AAA) |
| Angel Villalona | IF | 25.300 | San Francisco Giants | Richmond (AA) | Activated from disabled list |
| Julio Vivas | P | 22.251 | Pittsburgh Pirates | West Virginia (A) | Assigned to West Virginia (A) |
| Wei-Chung Wang | P | 24.045 | Milwaukee Brewers | Biloxi (AA) | Assigned to Biloxi (AA) |
| Austen Williams | P | 23.172 | Washington Nationals | Potomac (A+) | Assigned to Potomac (A+) |
| Kolten Wong | 2B | 25.242 | St. Louis Cardinals | Memphis (AAA) | Assigned to Memphis (AAA) |
| Mark Zagunis | C | 23.124 | Chicago Cubs | Iowa (AAA) | Assigned to Iowa (AAA) |
| Josh Zeid | RP | 29.077 | New York Mets | Binghamton (AA) | Assigned to Binghamton (AA) |
| Phil Coke | RP | 33.325 | New York Yankees | Scranton/Wilkes-Barre (AAA) | DL-7 Injured |
| Bryce Morrow | P | 28.159 | | | Released by Padres |
| Eric Stamets | IF | 24.258 | Cleveland Indians | Akron (AA) | DL-7 Injured |
| Isiah Kiner-Falefa | SS | 21.078 | Texas Rangers | Frisco (AA) | DL-7 Injured |
| Juan Santos | P | 20.284 | Houston Astros | Houston Astros (MLB) | Assigned to extended spring training |
| Chad Christensen | IF | 25.246 | | | Released by Twins |
| Josh Prevost | P | 24.146 | New York Mets | St. Lucie (A+) | DL-7 Injured |
| Joshua Uhen | P | 24.063 | Milwaukee Brewers | Brevard County (A+) | Placed on temporary inactive list |
| Oscar Mercado | SS | 21.175 | St. Louis Cardinals | Palm Beach (A+) | DL-7 Injured |
| Rodrigo Takahashi | P | 19.137 | Arizona Diamondbacks | Kane County (A) | DL-7 Injured |
| Steve Bean | C | 22.267 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Cristian Alvarado | P | 21.262 | Baltimore Orioles | Aberdeen (A-) | Assigned to Aberdeen (A-) |
| Jake Bray | P | 23.183 | Baltimore Orioles | Delmarva (A) | DL-7 Injured |
| Juan Pena | p | 20.289 | Colorado Rockies | Asheville (A) | DL-7 Injured |
| Robert Strader | p | 22.086 | Baltimore Orioles | Aberdeen (A-) | Assigned to Aberdeen (A-) |
| Zac Person | p | 23.239 | Houston Astros | Quad Cities (A) | DL-7 Injured |
