| Brett Cecil | RP | 27.352 | Toronto Blue Jays | Toronto Blue Jays (MLB) | DL-15 Groin strain |
| Kevin Gregg | RP | 35.364 | Miami Marlins | Miami Marlins (MLB) | Contract purchased from minors |
| Jarrod Saltalamacchia | c | 29.048 | Miami Marlins | Miami Marlins (MLB) | Activated from DL |
| Daniel Bard | RP | 28.359 | - | | Released by Rangers |
| Cameron Rupp | C | 25.264 | Philadelphia Phillies | Philadelphia Phillies (MLB) | Recalled from Lehigh Valley (AAA) |
| Daniel Hudson | P | 27.102 | Arizona Diamondbacks | Arizona Diamondbacks (MLB) | Transferred to 60-day DL |
| Matt Stites | P | 24.022 | Arizona Diamondbacks | Arizona Diamondbacks (MLB) | Contract purchased from minors |
| Yonder Alonso | 1b | 27.072 | San Diego Padres | San Diego Padres (MLB) | DL-15 Right wrist tendinitis |
| David DeJesus | lf | 34.182 | Tampa Bay Rays | Tampa Bay Rays (MLB) | DL-15 Left hand fracture |
| Brandon Guyer | LF | 28.142 | Tampa Bay Rays | Tampa Bay Rays (MLB) | Activated from DL |
| Eduardo Nunez | ss | 27.004 | Minnesota Twins | Minnesota Twins (MLB) | DL-15 Strained right hamstring |
| Yohan Pino | P | 30.176 | Minnesota Twins | Minnesota Twins (MLB) | Contract purchased from minors |
| Pedro Beato | RP | 27.235 | Atlanta Braves | Atlanta Braves (MLB) | DL-15 Right shoulder fatigue |
| Juan Jaime | P | 26.322 | Atlanta Braves | Atlanta Braves (MLB) | Recalled from Gwinnett (AAA) |
| Steven Delabar | RP | 30.338 | Toronto Blue Jays | Toronto Blue Jays (MLB) | Recalled from Buffalo (AAA) |
| Jake Goebbert | LF-RF | 26.269 | San Diego Padres | San Diego Padres (MLB) | Contract purchased from minors |
| Chris Nelson | 3b | 28.289 | San Diego Padres | San Diego Padres (MLB) | Signed to minor league contract |
| Anthony Alford | OF | 19.334 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Josh Almonte | OF | 20.142 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Jose Alonzo | | 21.115 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Aaron Altherr | LF-CF | 23.156 | Philadelphia Phillies | Reading (AA) | Assigned to Reading (AA) |
| Freddy Alvarez | P | 20.282 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Trae Arbet | SS | 19.353 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Martires Arias | P | 23.221 | New York Mets | Kingsport (Rk) | Assigned to Kingsport (Rk) |
| Daniel Arribas | | 21.262 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Agapito Barrios | | 20.201 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Frank Batista | P | 25.054 | Chicago Cubs | Tennessee (AA) | Assigned to Tennessee (AA) |
| Wuilmer Becerra | LF-RF | 19.261 | New York Mets | Kingsport (Rk) | Assigned to Kingsport (Rk) |
| Chad Bell | P | 25.111 | Texas Rangers | Myrtle Beach (A+) | Assigned to Myrtle Beach (A+) |
| Joe Benson | CF | 26.106 | Miami Marlins | New Orleans (AAA) | Assigned to New Orleans (AAA) |
| Cam Booser | P | 22.046 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Ryan Borucki | P | 20.080 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Andrew Brockett | P | 21.349 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Sean Buckley | 3B | 24.289 | Cincinnati Reds | Bakersfield (A+) | Activated from disabled list |
| Nick Buckner | CF | 18.315 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Ronald Bueno | | 21.258 | Cincinnati Reds | Dayton (A) | Assigned to Dayton (A) |
| Oscar Cabrera | P | 20.028 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Tony Campana | cf | 28.020 | Arizona Diamondbacks | Reno (AAA) | Assigned to Reno (AAA) |
| Jeimer Candelario | | 20.207 | Chicago Cubs | Kane County (A) | Assigned to Kane County (A) |
| Malquin Canelo | SS | 19.287 | Philadelphia Phillies | Lakewood (A) | Assigned to Lakewood (A) |
| Cristian Cano | | 20.130 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Pat Cantwell | C | 24.070 | Texas Rangers | Frisco (AA) | Activated from disabled list |
| Hunter Carnevale | P | 25.296 | New York Mets | Savannah (A) | Assigned to Savannah (A) |
| Damion Carroll | P | 20.139 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Yeiper Castillo | P | 25.284 | Oakland Athletics | Stockton (A+) | Assigned to Stockton (A+) |
| Jose Ceballos | C | 25.174 | Miami Marlins | Greensboro (A) | Assigned to Greensboro (A) |
| Gavin Cecchini | SS | 20.179 | New York Mets | St. Lucie (A+) | Assigned to St. Lucie (A+) |
| Gabriel Cenas | | 20.246 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Gerald Chin | 2B | 21.021 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| TJ Chism | P | 25.314 | New York Mets | St. Lucie (A+) | Assigned to St. Lucie (A+) |
| Joe Church | P | 24.263 | San Diego Padres | Lake Elsinore (A+) | Assigned to Lake Elsinore (A+) |
| Brett Clements | | 23.269 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Robby Coles | P | 22.304 | New York Mets | St. Lucie (A+) | Assigned to St. Lucie (A+) |
| Cale Coshow | P | 21.338 | New York Yankees | Charleston (sc) (A) | Assigned to Charleston (Sc) (A) |
| JP Crawford | SS | 19.159 | Philadelphia Phillies | Clearwater (A+) | Assigned to Clearwater (A+) |
| Victor Cruzado | OF | 22.016 | New York Mets | Savannah (A) | Activated from disabled list |
| Ryan Dale | | 18.095 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Chase Darhower | SS | 21.158 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Mervin Del Rosario | | 22.096 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Matt Den Dekker | CF | 26.314 | New York Mets | Las Vegas (AAA) | Assigned to Las Vegas (AAA) |
| Andrew Dennis | C | 21.081 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Dutch Deol | LF | 21.242 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Hunter Dozier | SS | 22.302 | Kansas City Royals | Northwest Arkansas (AA) | Assigned to Northwest Arkansas (AA) |
| Taylor Dugas | OF | 24.186 | New York Yankees | Scranton/Wilkes-Barre (AAA) | Assigned to Scranton/Wilkes-Barre (AAA) |
| Brandon Dulin | 1B | 21.172 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Nolan Earley | OF | 23.084 | Chicago White Sox | Kannapolis (A) | Assigned to Kannapolis (A) |
| Sebastian Elizalde | OF | 22.212 | Cincinnati Reds | Bakersfield (A+) | Assigned to Bakersfield (A+) |
| Jeffery Enloe | P | 24.310 | San Diego Padres | Fort Wayne (A) | Assigned to Fort Wayne (A) |
| Jonathan Escudero | | 20.347 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Ariel Estades | OF | 20.053 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Steven Farinaro | P | 18.306 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Jorge Fernandez | C | 20.081 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Xavier Fernandez | C | 18.340 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Arby Fields | OF | 22.360 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Christian Flecha | P | 19.041 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Randy Fontanez | P | 25.032 | New York Mets | Binghamton (AA) | Assigned to Binghamton (AA) |
| Hyrum Formo | P | 22.067 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Parker Frazier | P | 25.220 | Chicago White Sox | Charlotte (AAA) | Assigned to Charlotte (AAA) |
| Amalani Fukofuka | CF | 18.268 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Jeff Gelalich | OF | 23.095 | Cincinnati Reds | Bakersfield (A+) | Assigned to Bakersfield (A+) |
| Darryl George | 1B | 21.097 | Tampa Bay Rays | Bowling Green (A) | Activated from disabled list |
| Devonte German | P | 19.248 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Sam Gibbons | | 20.189 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Jose Gomez | | 22.141 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Carlos Gonzalez | P | 24.007 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) |
| Trevor Gott | P | 21.297 | San Diego Padres | San Antonio (AA) | Assigned to San Antonio (AA) |
| Matt Grace | P | 25.187 | Washington Nationals | Syracuse (AAA) | Assigned to Syracuse (AAA) |
| Scott Gracey | P | 27.247 | Toronto Blue Jays | New Hampshire (AA) | Activated from disabled list |
| Alec Grosser | P | 19.158 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Reymin Guduan | P | 22.095 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Ismael Guillon | P | 22.127 | Cincinnati Reds | Bakersfield (A+) | Assigned to Bakersfield (A+) |
| Joey Gunkel | P | 22.172 | Boston Red Sox | Salem (A+) | Assigned to Salem (A+) |
| Jordan Hankins | IF-C | 22.122 | Chicago Cubs | Daytona (A+) | Assigned to Daytona (A+) |
| David Harris | SS | 22.314 | Toronto Blue Jays | Lansing (A) | Assigned to Lansing (A) |
| Taylor Hawkins | C | 20.275 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Adam Heisler | CF | 26.012 | Chicago White Sox | Winston-Salem (A+) | Activated from disabled list |
| Lucas Herbst | OF-P | 23.283 | Baltimore Orioles | Delmarva (A) | Assigned to Delmarva (A) |
| Dilson Herrera | 2B | 20.108 | New York Mets | Binghamton (AA) | Assigned to Binghamton (AA) |
| Josh Hodges | P | 22.364 | Miami Marlins | Batavia (A-) | Assigned to Batavia (A-) |
| Colby Holmes | P | 23.238 | Atlanta Braves | Rome (A) | Assigned to Rome (A) |
| Carter Hope | P | 19.134 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Chih-Wei Hu | | 20.227 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Will Hurt | SS | 20.179 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Danny Jansen | C | 19.065 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Myles Jaye | P | 22.174 | Chicago White Sox | Birmingham (AA) | Assigned to Birmingham (AA) |
| Dan Jennings | RP | 27.063 | Miami Marlins | New Orleans (AAA) | Assigned to New Orleans (AAA) |
| Coco Johnson | OF | 23.047 | Miami Marlins | Greensboro (A) | Assigned to Greensboro (A) |
| Felix Jorge | P | 20.168 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Ryan Kelly | P | 26.233 | Atlanta Braves | Mississippi (AA) | Assigned to Mississippi (AA) |
| Matt Kimbrel | P | 24.098 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Riley King | CF | 20.057 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Dan Klein | C | 23.294 | Toronto Blue Jays | Lansing (A) | Assigned to Lansing (A) |
| Jared Lakind | 1B | 22.102 | Pittsburgh Pirates | Jamestown (A-) | Assigned to Jamestown (A-) |
| Mitch Lambson | P | 23.334 | Houston Astros | Corpus Christi (AA) | Assigned to Corpus Christi (AA) |
| Emerson Landoni | 2B | 25.120 | Atlanta Braves | Lynchburg (A+) | Assigned to Lynchburg (A+) |
| Luis Lara | C | 19.136 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Daniel Lietz | P | 20.018 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Zack Littell | P | 18.258 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Ben Lively | P | 22.106 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) |
| Tyler Lyons | SP | 26.119 | St. Louis Cardinals | Memphis (AAA) | Activated from DL and assigned to Memphis (AAA) |
| Kevin Mager | 1b | 25.034 | Boston Red Sox | Greenville (A) | Assigned to Greenville (A) |
| Beau Maggi | C | 23.220 | Kansas City Royals | Wilmington (A+) | Assigned to Wilmington (A+) |
| Trey Mancini | 1B | 22.093 | Baltimore Orioles | Frederick (A+) | Assigned to Frederick (A+) |
| Felix Marte | RF | 23.217 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Ydarqui Marte | LF | 21.252 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Jason Martin | CF | 18.288 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Dailyn Martinez | P | 21.061 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Luis Mateo | 2B | 24.027 | St. Louis Cardinals | Springfield (AA) | Assigned to Springfield (AA) |
| Kevin Mattison | CF | 28.272 | Milwaukee Brewers | Nashville (AAA) | Assigned to Nashville (AAA) |
| Steven Matz | P | 23.021 | New York Mets | Binghamton (AA) | Assigned to Binghamton (AA) |
| Mike Mayers | P | 22.196 | St. Louis Cardinals | Springfield (AA) | Assigned to Springfield (AA) |
| LJ Mazzilli | IF | 23.286 | New York Mets | St. Lucie (A+) | Assigned to St. Lucie (A+) |
| Kevin McGowan | P | 22.245 | New York Mets | St. Lucie (A+) | Assigned to St. Lucie (A+) |
| Ian McKinney | p | 19.213 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Jeff McNeil | IF-OF | 22.072 | New York Mets | St. Lucie (A+) | Assigned to St. Lucie (A+) |
| Jeffeson Medina | | 20.019 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Martin Medina | 1B-C | 24.087 | Chicago White Sox | Birmingham (AA) | Assigned to Birmingham (AA) |
| Yonathan Mejia | 2B | 21.273 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Seth Mejias-Brean | IF | 23.075 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) |
| Oscar Mercado | SS | 19.185 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Brandon Meredith | LF | 24.182 | Houston Astros | Lancaster (A+) | Activated from disabled list |
| Thomas Milone | OF | 19.144 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Daniel Missaki | | 18.071 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Luis Monasterio | | 19.220 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Josue Montanez | P | 22.156 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Hector Montes | 1B | 22.119 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Ulises Montilla | | 22.038 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Estarlyn Morales | | 21.234 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Tomas Morales | C | 22.325 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Julio Morban | CF-LF | 22.127 | Seattle Mariners | Jackson (AA) | Activated from disabled list |
| Julio Morillo | C | 21.174 | Cincinnati Reds | Bakersfield (A+) | Assigned to Bakersfield (A+) |
| Conor Mullee | P | 26.115 | New York Yankees | Charleston (sc) (A) | Activated from disabled list |
| Jairo Munoz | | 22.312 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Tanner Murphy | C | 19.112 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Omar Narvaez | C-1B | 22.130 | Chicago White Sox | Winston-Salem (A+) | Assigned to Winston-Salem (A+) |
| Brian Navarreto | C | 19.172 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Charlie Neil | OF | 24.138 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Chris Nelson | 3b | 28.289 | San Diego Padres | El Paso (AAA) | Assigned to El Paso (AAA) |
| Grant Nelson | C-3B | 22.285 | Arizona Diamondbacks | South Bend (A) | Assigned to South Bend (A) |
| Austin Nicely | P | 19.188 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Brandon Nimmo | CF | 21.084 | New York Mets | Binghamton (AA) | Assigned to Binghamton (AA) |
| Jacob Nottingham | C | 19.077 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Mikey O'Brien | P | 24.108 | Cincinnati Reds | Bakersfield (A+) | Assigned to Bakersfield (A+) |
| Omar Obregon | | 20.062 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Mikeson Oliberto | OF | 23.300 | New York Yankees | Charleston (sc) (A) | Assigned to Charleston (Sc) (A) |
| Jose Ortiz | C | 20.008 | Cincinnati Reds | Dayton (A) | Assigned to Dayton (A) |
| Carlos Ozuna | | 20.335 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Jose Paez | | 20.312 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Alexy Palma | | 21.177 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Alex Panteliodis | P | 23.347 | New York Mets | Savannah (A) | Assigned to Savannah (A) |
| Ruben Paredes | | 20.271 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Derrick Penilla | P | 22.171 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Jose Peraza | | 20.050 | Atlanta Braves | Mississippi (AA) | Assigned to Mississippi (AA) |
| Georvic Perez | | 19.065 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Jorge Perez | P | 20.324 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Juan Perez | P | 18.333 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Tim Peterson | P | 23.117 | New York Mets | St. Lucie (A+) | Assigned to St. Lucie (A+) |
| Tyler Pike | P | 20.144 | Seattle Mariners | Jackson (AA) | Assigned to Jackson (AA) |
| Cesilio Pimentel | P | 21.165 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Jeremias Pineda | CF | 23.215 | Minnesota Twins | Elizabethton (Rk) | Activated from disabled list |
| Carlos Ramirez | OF | 23.056 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Roel Ramirez | P | 19.024 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| David Ramos | P | 22.280 | Washington Nationals | Hagerstown (A) | Assigned to Hagerstown (A) |
| Anthony Ray | CF | 19.108 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| J.T. Realmuto | C | 23.093 | Miami Marlins | Jacksonville (AA) | Assigned to Jacksonville (AA) |
| Frankie Reed | P | 26.128 | Miami Marlins | Jacksonville (AA) | Assigned to Jacksonville (AA) |
| Hunter Renfroe | C | 22.143 | San Diego Padres | San Antonio (AA) | Assigned to San Antonio (AA) |
| Matt Reynolds | IF | 23.198 | New York Mets | Las Vegas (AAA) | Assigned to Las Vegas (AAA) |
| Jonathan Reynoso | | 21.163 | Cincinnati Reds | Dayton (A) | Assigned to Dayton (A) |
| Josh Richardson | P | 22.323 | San Diego Padres | Fort Wayne (A) | Assigned to Fort Wayne (A) |
| Chris Rivera | SS | 19.101 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Maximo Rivera | | 21.179 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| TJ Rivera | 2B | 25.235 | New York Mets | Binghamton (AA) | Assigned to Binghamton (AA) |
| Tim Roberson | C | 24.335 | Boston Red Sox | Greenville (A) | Assigned to Greenville (A) |
| Jairo Rodriguez | C | 25.299 | Minnesota Twins | Fort Myers (A+) | Assigned to Fort Myers (A+) |
| Billy Roth | P | 19.014 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Yeixon Ruiz | | 23.092 | New York Mets | Savannah (A) | Assigned to Savannah (A) |
| Sam Runion | P | 25.222 | Washington Nationals | Potomac (A+) | Assigned to Potomac (A+) |
| Angel Sanchez | | 21.109 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Fernelys Sanchez | OF | 20.110 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Jake Sanchez | P | 24.304 | Oakland Athletics | Stockton (A+) | Assigned to Stockton (A+) |
| Jonathan Sandfort | P | 19.296 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Juan Santos | P | 18.294 | Houston Astros | Greeneville (Rk) | Assigned to Greeneville (Rk) |
| Troy Scott | P | 20.214 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Rolando Segovia | | 19.236 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Isaac Silva | | 21.280 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Creede Simpson | SS | 24.284 | Baltimore Orioles | Delmarva (A) | Assigned to Delmarva (A) |
| Marquez Smith | 3B | 29.091 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) |
| Matt Smoral | P | 20.093 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Ariel Soriano | 2B-SS | 23.207 | Tampa Bay Rays | Charlotte (A+) | Assigned to Charlotte (A+) |
| Jackson Stephens | P | 20.039 | Cincinnati Reds | Dayton (A) | Assigned to Dayton (A) |
| Ian Stiffler | P | 19.127 | Atlanta Braves | Danville (Rk) | Assigned to Danville (Rk) |
| Tyler Stirewalt | P | 23.195 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Matt Strahm | P | 22.220 | Kansas City Royals | Lexington (A) | Assigned to Lexington (A) |
| Carlton Tanabe | C | 22.235 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Rowdy Tellez | 1B | 19.095 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Christopher Thomas | P | 26.095 | St. Louis Cardinals | Springfield (AA) | Assigned to Springfield (AA) |
| Ismael Tijerina | SS-2B | 24.304 | New York Mets | Kingsport (Rk) | Assigned to Kingsport (Rk) |
| Jesus Tinoco | P | 19.050 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Cristian Toribio | SS | 19.279 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Carlos Torres | OF | 21.261 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Daniel Torres | C | 22.021 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Ryan Tufts | OF | 25.138 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
| Riley Unroe | SS | 18.321 | Tampa Bay Rays | Princeton (Rk) | Assigned to Princeton (Rk) |
| Ugueth Urbina | | 19.234 | Seattle Mariners | Pulaski (Rk) | Assigned to Pulaski (Rk) |
| Patrick Urckfitz | P | 25.333 | Houston Astros | Oklahoma City (AAA) | Assigned to Oklahoma City (AAA) |
| Richard Urena | SS | 18.114 | Toronto Blue Jays | Bluefield (Rk) | Assigned to Bluefield (Rk) |
| Jesus Ustariz | 1b | 21.054 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Sebastian Valle | C | 23.330 | Philadelphia Phillies | Lehigh Valley (AAA) | Assigned to Lehigh Valley (AAA) |
| Enyel Vallejo | SS | 23.247 | Pittsburgh Pirates | Bristol (Rk) | Assigned to Bristol (Rk) |
| Ben Verlander | P | 22.140 | Detroit Tigers | Connecticut (A-) | Assigned to Connecticut (A-) |
| Christian Villanueva | 3B | 23.000 | Chicago Cubs | Tennessee (AA) | Assigned to Tennessee (AA) |
| Kender Villegas | | 21.011 | St. Louis Cardinals | Johnson City (Rk) | Assigned to Johnson City (Rk) |
| Arodys Vizcaino | P | 23.218 | Chicago Cubs | Iowa (AAA) | Assigned to Iowa (AAA) |
| Kyle Waldrop | OF | 22.206 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) |
| Jordan Weems | C | 21.224 | Boston Red Sox | Salem (A+) | Assigned to Salem (A+) |
| Jason Wheeler | P | 23.235 | Minnesota Twins | New Britain (AA) | Assigned to New Britain (AA) |
| Stephen Wickens | 2B-SS | 25.106 | Minnesota Twins | New Britain (AA) | Assigned to New Britain (AA) |
| Ty Williams | P | 20.118 | New York Mets | Kingsport (Rk) | Assigned to Kingsport (Rk) |
| Jesse Winker | OF | 20.306 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) |
| Jantzen Witte | IF | 24.166 | Boston Red Sox | Salem (A+) | Assigned to Salem (A+) |
| Ryan Wright | 2B | 24.198 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) |
| Jimmy Yacabonis | P | 22.090 | Baltimore Orioles | Frederick (A+) | Assigned to Frederick (A+) |
| Nelfi Zapata | C | 23.188 | New York Mets | Savannah (A) | Assigned to Savannah (A) |
| Mike Pelfrey | SP | 30.157 | Minnesota Twins | Minnesota Twins (MLB) | Transferred to 60-day DL |
| Argenis Diaz | SS | 27.127 | | | Released by Reds |
| Brent Lillibridge | 3b | 30.275 | Texas Rangers | Round Rock (AAA) | DL-7 Injured |
| Ryan Mattheus | RP | 30.222 | Washington Nationals | Syracuse (AAA) | DL-7 Injured |
| Sean Nolin | SP | 24.175 | Toronto Blue Jays | Buffalo (AAA) | DL-7 Injured |
| Shelley Duncan | dh | 34.264 | | | Released by Reds |
| Chih-Hsien Chiang | RF | 26.119 | Baltimore Orioles | Bowie (AA) | DL-7 Injured |
| Dallas Tarleton | C | 26.319 | Arizona Diamondbacks | Reno (AAA) | DL-7 Injured |
| Destin Hood | RF | 24.077 | Washington Nationals | Syracuse (AAA) | DL-7 Injured |
| Edison Frias | P | 23.183 | Houston Astros | Quad Cities (A) | Assigned to Quad Cities (A) |
| Wade Kirkland | SS-3B | 25.076 | Oakland Athletics | Stockton (A+) | Assigned to Stockton (A+) |
| Stetson Allie | 3B | 23.098 | Pittsburgh Pirates | Altoona (AA) | DL-7 Injured |
| Alberto Diaz | P | 23.007 | Chicago Cubs | Boise (A-) | Assigned to Boise (A-) |
| John Means | P | 21.056 | Baltimore Orioles | Aberdeen (A-) | Assigned to Aberdeen (A-) |
| Ethan Wilson | IF | 25.102 | Chicago White Sox | Great Falls (Rk) | DL-7 Injured |
| Aaron Brown | RF | 21.364 | Philadelphia Phillies | Williamsport (A-) | Signed out of draft (Pepperdine) |
| Austin Pettibone | P | 21.282 | Texas Rangers | Spokane (A-) | Signed out of draft (UC Santa Barbara) |
| Bradley Zimmer | CF | 21.204 | Cleveland Indians | Mahoning Valley (A-) | Signed out of draft (San Francisco) |
| Erik Forgione | SS | 21.283 | Pittsburgh Pirates | Jamestown (A-) | Signed out of draft (Washington) |
| Mason McCullough | P | 21.163 | Arizona Diamondbacks | Hillsboro (A-) | Signed out of draft (Lander) |
| Sam Travis | 3B | 20.296 | Boston Red Sox | Lowell (A-) | Signed out of draft (Indiana) |
| Jake Raffaele | P | 23.116 | Texas Rangers | Arizona Rangers (Rk) | Signed as undrafted free agent |
| Josciel Veras | SS | 21.194 | Cincinnati Reds | Billings (Rk) | Signed out of draft (Cumberland) |
| Justin Anderson | P | 21.264 | Los Angeles Angels | Orem (Rk) | Signed out of draft (Texas-San Antonio) |
| Patrick Armstrong | IF | 22.339 | Los Angeles Angels | Arizona Angels (Rk) | Signed out of draft (UNLV) |
| Richard Gonzalez | C | 20.213 | Houston Astros | Gulf Coast Astros (Rk) | Signed out of draft (Alabama State) |
| Trevor Oaks | P | 21.085 | Los Angeles Dodgers | Ogden (Rk) | Signed out of draft (California Baptist) |
| Tim Saunders | SS | 24.033 | | | Announced retirement |
| Welington Castillo | C | 27.056 | Chicago Cubs | Iowa (AAA) | Assigned to Iowa (AAA) for rehab |
| J.T. Chargois | P | 23.198 | Minnesota Twins | Elizabethton (Rk) | Assigned to Elizabethton (Rk) |
