| Eric Hinske | 1b | 35.348 | | | Released by Diamondbacks |
| Chris Getz | 2b | 29.324 | Kansas City Royals | Kansas City Royals (MLB) | Recalled from Omaha (AAA) |
| Kelvin Herrera | RP | 23.200 | Kansas City Royals | Kansas City Royals (MLB) | Recalled from Northwest Arkansas (AA) |
| Jeff Baker | 1b | 32.028 | Texas Rangers | Texas Rangers (MLB) | Activated from DL |
| Craig Gentry | lf-cf | 29.233 | Texas Rangers | Texas Rangers (MLB) | Activated from DL |
| Hector Ambriz | RP | 29.051 | Houston Astros | Houston Astros (MLB) | Recalled from Oklahoma City (AAA) |
| Fernando Salas | RP | 28.050 | St. Louis Cardinals | St. Louis Cardinals (MLB) | Recalled from Memphis (AAA) |
| James Loney | 1b | 29.073 | Tampa Bay Rays | Tampa Bay Rays (MLB) | Placed on Paternity Leave list |
| Ryan Roberts | 2b | 32.303 | Tampa Bay Rays | Tampa Bay Rays (MLB) | Recalled from Durham (AAA) |
| Chris Carpenter | SP | 38.083 | St. Louis Cardinals | Memphis (AAA) | Assigned to Memphis (AAA) for rehab |
| Franklin Gutierrez | cf | 30.148 | Seattle Mariners | Tacoma (AAA) | Assigned to Tacoma (AAA) for rehab |
| Stephen Pryor | RP | 23.361 | Seattle Mariners | Tacoma (AAA) | Assigned to Tacoma (AAA) for rehab |
| Wilmer Flores | 3B | 21.348 | New York Mets | Las Vegas (AAA) | DL-7 Injured |
| Scott Moore | 3B | 29.245 | San Diego Padres | Tucson (AAA) | Signed to minor league contract |
| Shawn Camp | RP | 37.244 | Arizona Diamondbacks | Reno (AAA) | Signed to minor league contract |
| Esmailin Caridad | P | 29.265 | | | Released by Cubs |
| Jose Valdez | P | 30.178 | | | Released by Astros |
| Jake Jefferies | C | 25.263 | | | Announced retirement |
| Brayan Villarreal | RP | 26.070 | Detroit Tigers | Toledo (AAA) | DL-7 Injured |
| Atahualpa Severino | P | 28.255 | Pittsburgh Pirates | Indianapolis (AAA) | Activated from temporarily inactive list |
| J.C. Linares | LF-RF | 28.315 | Boston Red Sox | Pawtucket (AAA) | DL-7 Injured |
| Jose Valverde | RP | 35.117 | Detroit Tigers | Toledo (AAA) | Activated from temporarily inactive list |
| Xander Bogaerts | SS | 20.291 | Boston Red Sox | Pawtucket (AAA) | Activated from temporarily inactive list |
| Jeff Gray | P | 31.242 | | | Released by White Sox |
| Sean Nicol | 3B | 26.297 | Washington Nationals | Harrisburg (AA) | DL-7 Injured |
| Chris Wallace | C | 25.083 | Cleveland Indians | Akron (AA) | DL-7 Injured |
| Chris Balcom-Miller | P | 24.138 | Boston Red Sox | Portland (AA) | DL-7 Injured |
| Sandy Leon | c | 24.128 | Washington Nationals | Harrisburg (AA) | DL-7 Injured |
| Paul Konerko | dh | 37.136 | Chicago White Sox | Birmingham (AA) | Assigned to Birmingham (AA) for rehab |
| Riccio Torrez | 3B | 23.278 | Tampa Bay Rays | Montgomery (AA) | DL-7 Left hand |
| Jordan Conley | P | 27.000 | | | Released by Marlins |
| Brandon Short | CF-LF | 24.313 | Chicago White Sox | Birmingham (AA) | DL-7 Injured |
| Brian Smith | P | 20.219 | Chicago Cubs | Kane County (A) | DL-7 Injured |
| Terrell Joyce | OF | 21.051 | Houston Astros | Quad Cities (A) | DL-7 Injured |
| Zak Adams | P | 21.122 | | | Released by Blue Jays |
| Destin Hood | RF | 23.107 | Washington Nationals | Harrisburg (AA) | Activated from DL |
| Brian Ward | C | 27.275 | Baltimore Orioles | Bowie (AA) | Activated from DL |
| Bo Altobelli | C | 22.163 | Minnesota Twins | Elizabethton (Rk) | Activated from DL |
| Dixon Llorens | P | 20.243 | St. Louis Cardinals | Peoria (A) | Activated from DL |
| Chris Colabello | rf | 29.269 | Minnesota Twins | Minnesota Twins (MLB) | Recalled from Rochester (AAA) |
| Derek Jeter | dh | 39.023 | New York Yankees | New York Yankees (MLB) | DL-15 Strained right quad [Running out a grounder] |
| Brent Lillibridge | 3b | 29.305 | New York Yankees | New York Yankees (MLB) | Contract purchased from Scranton/Wilkes-Barre (AAA) |
| Jake Peavy | SP | 32.049 | Chicago White Sox | Chicago White Sox (MLB) | Activated from DL |
| Brian Bogusevic | cf | 29.152 | Chicago Cubs | Chicago Cubs (MLB) | DL-15 Left hamstring strain [Chaing a ball in outfield] |
| Junior Lake | cf | 23.114 | Chicago Cubs | Chicago Cubs (MLB) | Recalled from Iowa (AAA) |
| Henry Rodriguez | RP | 26.144 | Chicago Cubs | Iowa (AAA) | Outrighted to Iowa (AAA) |
| Aaron Cook | P | 34.161 | | | Released by Rockies |
| Pedro Ciriaco | 2b | 27.295 | Kansas City Royals | Omaha (AAA) | Outrighted to Omaha (AAA) |
| Chris Dickerson | dh | 31.100 | Baltimore Orioles | Baltimore Orioles (MLB) | Designated for Assignment |
| Nolan Reimold | dh | 29.281 | Baltimore Orioles | Baltimore Orioles (MLB) | DL-15 Nerve inflammation in cervical spine [Related to neck injury last season] |
| Henry Urrutia | dh | 26.156 | Baltimore Orioles | Baltimore Orioles (MLB) | Contract purchased from Norfolk (AAA) |
| Danny Valencia | dh | 28.303 | Baltimore Orioles | Baltimore Orioles (MLB) | Recalled from Norfolk (AAA) |
| Andrew Bailey | RP | 29.049 | Boston Red Sox | Boston Red Sox (MLB) | DL-15 Right shoulder strain |
| Jose De La Torre | RP | 27.275 | Boston Red Sox | Boston Red Sox (MLB) | Recalled from Pawtucket (AAA) |
| Brennan Boesch | rf | 28.098 | | | Released by Yankees |
| Ronny Paulino | C | 32.089 | Baltimore Orioles | Norfolk (AAA) | Assigned to Norfolk (AAA) |
| Chris Gutierrez | SS | 29.129 | Miami Marlins | Jacksonville (AA) | Assigned to Jacksonville (AA) |
| Justin Turner | ss | 28.238 | New York Mets | Brooklyn (A-) | Assigned to Brooklyn (A-) |
| Melky Cabrera | lf | 28.342 | Toronto Blue Jays | Buffalo (AAA) | Assigned to Buffalo (AAA) for rehab |
| Josh Vitters | 3b | 23.326 | Chicago Cubs | Iowa (AAA) | Assigned to Iowa (AAA) |
| Oscar Tejeda | 2B | 23.205 | Pittsburgh Pirates | Altoona (AA) | Assigned to Altoona (AA) |
| Carson Blair | C | 23.274 | Boston Red Sox | Lowell (A-) | Assigned to Lowell (A-) |
| Zack Cox | 3B | 24.071 | Miami Marlins | Jacksonville (AA) | Assigned to Jacksonville (AA) |
| Micah Gibbs | C | 24.357 | Kansas City Royals | Wilmington (A+) | Contract purchased from Grand Prairie (American) |
| Hector Crespo | IF | 21.324 | New York Yankees | Scranton/Wilkes-Barre (AAA) | Assigned to Scranton/Wilkes-Barre (AAA) |
| Jose Colorado | RF | 22.327 | Boston Red Sox | Lowell (A-) | Assigned to Lowell (A-) |
| Chad Carman | C | 24.071 | Philadelphia Phillies | Lakewood (A) | Assigned to Lakewood (A) |
| Lewis Urena | 2B | 22.074 | Kansas City Royals | Idaho Falls (Rk) | Assigned to Idaho Falls (Rk) |
| Beau Wallace | 3B | 20.356 | Pittsburgh Pirates | Gulf Coast Pirates (Rk) | Assigned to Gulf Coast Pirates (Rk) |
| John Riley | C | 19.155 | San Francisco Giants | Arizona Giants (Rk) | |
| Jose Contreras | RP | 41.226 | Boston Red Sox | Pawtucket (AAA) | Signed to minor league contract |
| Carlos Marmol | RP | 30.278 | Los Angeles Dodgers | Chattanooga (AA) | Assigned to Chattanooga (AA) |
| Jon Link | P | 29.118 | Detroit Tigers | Toledo (AAA) | Assigned to Toledo (AAA) |
| Mark Serrano | P | 27.308 | Arizona Diamondbacks | Mobile (AA) | Assigned to Mobile (AA) |
| Dae-Eun Rhee | P | 24.118 | Chicago Cubs | Tennessee (AA) | Assigned to Tennessee (AA) |
| Matt Sample | P | 24.337 | Arizona Diamondbacks | South Bend (A) | Assigned to South Bend (A) |
| Brad Hand | P | 23.121 | Miami Marlins | Gulf Coast Marlins (Rk) | Assigned to Gulf Coast Marlins (Rk) |
| Scott McGough | P | 23.261 | Miami Marlins | Jacksonville (AA) | Assigned to Jacksonville (AA) |
| Gregory Billo | P | 23.004 | Kansas City Royals | Lexington (A) | Assigned to Lexington (A) |
| Jay Johnson | P | 23.210 | Philadelphia Phillies | Lehigh Valley (AAA) | Assigned to Lehigh Valley (AAA) |
| Keith Butler | RP | 24.170 | St. Louis Cardinals | Memphis (AAA) | Assigned to Memphis (AAA) |
| JD Osborne | P | 22.248 | Houston Astros | Tri City (A-) | Assigned to Tri City (A-) |
| Trent Howard | P | 23.276 | Baltimore Orioles | Bowie (AA) | Assigned to Bowie (AA) |
| Steven Gruver | P | 24.019 | Minnesota Twins | Fort Myers (A+) | Assigned to Fort Myers (A+) |
| Leonel Santiago | P | 23.208 | Kansas City Royals | Wilmington (A+) | Assigned to Wilmington (A+) |
| Nick Vander Tuig | P | 21.223 | San Francisco Giants | Arizona Giants (Rk) | |
| Tyler Jones | P | 23.317 | Minnesota Twins | Fort Myers (A+) | Assigned to Fort Myers (A+) |
| Augey Bill | P | 22.119 | Baltimore Orioles | Aberdeen (A-) | Assigned to Aberdeen (A-) |
| Jon Prosinski | P | 22.152 | Philadelphia Phillies | Lakewood (A) | Assigned to Lakewood (A) |
| Carlos Contreras | P | 22.192 | Cincinnati Reds | Pensacola (AA) | Assigned to Pensacola (AA) |
| Deyvi Jimenez | P | 23.201 | Oakland Athletics | Vermont (A-) | Assigned to Vermont (A-) |
| Brian Holmes | P | 22.170 | Houston Astros | Gulf Coast Astros (Rk) | Assigned to Gulf Coast Astros (Rk) |
| Yoimer Camacho | P | 23.145 | Arizona Diamondbacks | South Bend (A) | Assigned to South Bend (A) |
| Bruedlin Suero | P | 23.141 | Tampa Bay Rays | Gulf Coast Rays (Rk) | Assigned to Gulf Coast Rays (Rk) for rehab |
| Geisel De La Cruz | | 20.099 | Tampa Bay Rays | Gulf Coast Rays (Rk) | Assigned to Gulf Coast Rays (Rk) |
| Hunter Harvey | P | 18.222 | Baltimore Orioles | Gulf Coast Orioles (Rk) | |
| Max Beatty | P | 22.114 | San Diego Padres | Arizona Padres (Rk) | Assigned to Arizona Padres (Rk) |
| Junior Rincon | | 21.225 | Miami Marlins | Gulf Coast Marlins (Rk) | Assigned to Gulf Coast Marlins (Rk) |
| Daniel McGrath | | 19.012 | Boston Red Sox | Lowell (A-) | Assigned to Lowell (A-) |
| Luis Rico | P | 19.232 | Kansas City Royals | Arizona Royals (Rk) | |
| Brandon Lyon | RP | 33.344 | Boston Red Sox | Pawtucket (AAA) | Signed to minor league contract |
| Logan Brumley | 2B-SS | 24.046 | | | Signed by Southern Illinois (Frontier) |
| Tony Rizzotti | P | 21.089 | San Diego Padres | Arizona Padres (Rk) | Signed out of draft (Tulane) |
