| Gavin Floyd | SP | 29.177 | Chicago White Sox | Chicago White Sox (MLB) | Activated from DL |
| Ian Desmond | ss | 26.306 | Washington Nationals | Washington Nationals (MLB) | DL-15 Torn left oblique |
| Corey Brown | cf | 26.239 | Washington Nationals | Washington Nationals (MLB) | Recalled from Syracuse (AAA) |
| Jayson Werth | RF | 33.064 | Washington Nationals | Syracuse (AAA) | Assigned to Syracuse (AAA) for rehab |
| Chad Tracy | 3b-1b | 32.062 | Washington Nationals | Syracuse (AAA) | Assigned to Syracuse (AAA) for rehab |
| Chien-Ming Wang | RP-SP | 32.114 | Washington Nationals | Harrisburg (AA) | Assigned to Harrisburg (AA) for rehab |
| Brent Keys | CF | 22.009 | Miami Marlins | Greensboro (A) | Activated from DL |
| Wes Wilson | C | 22.339 | New York Yankees | Staten Island (A-) | DL-7 Injured |
| Dennis Torres | P | 22.067 | Baltimore Orioles | Gulf Coast Orioles (Rk) | DL-60 Injured |
| Andury Acevedo | SS | 21.334 | New York Yankees | Gulf Coast Yankees (Rk) | DL-60 Injured |
| Fernando Perez | SS | 21.226 | New York Yankees | Gulf Coast Yankees (Rk) | DL-60 Injured |
| Victor Beriguete | P | 23.259 | New York Yankees | Gulf Coast Yankees (Rk) | DL-60 Injured |
| Anthony Varvaro | RP | 27.265 | Atlanta Braves | Gwinnett (AAA) | Optioned to Gwinnett (AAA) |
| Jose Constanza | lf | 28.326 | Atlanta Braves | Atlanta Braves (MLB) | Recalled from Gwinnett (AAA) |
| Matt Nevarez | P | 25.147 | Tampa Bay Rays | Charlotte (A+) | Contract purchased from Wichita (Indy) |
| Colby Lewis | SP | 32.356 | Texas Rangers | Texas Rangers (MLB) | DL-15 Right elbow tendinitis |
| Martin Perez | RP-SP | 21.110 | Texas Rangers | Texas Rangers (MLB) | Recalled from Round Rock (AAA) |
| Omar Infante | 2b | 30.209 | Detroit Tigers | Detroit Tigers (MLB) | Traded from Marlins with Anibal Sanchez to Tigers for Jacob Turner, Rob Brantly and Brian Flynn |
| Anibal Sanchez | SP | 28.147 | Detroit Tigers | Detroit Tigers (MLB) | Traded from Marlins with Omar Infante to Tigers for Jacob Turner, Rob Brantly and Brian Flynn |
| Jacob Turner | SP | 21.063 | Miami Marlins | Miami Marlins (MLB) | Traded from Tigers with Rob Brantly and Brian Flynn to Marlins for Anibal Sanchez and Omar Infante |
| Chad Billingsley | SP | 27.359 | Los Angeles Dodgers | Los Angeles Dodgers (MLB) | Activated from DL |
| Josh Wall | RP | 25.183 | Los Angeles Dodgers | Albuquerque (AAA) | Optioned to Albuquerque (AAA) |
| Dewayne Wise | lf-rf | 34.149 | New York Yankees | New York Yankees (MLB) | Designated for assignment |
| DJ Mitchell | RP | 25.071 | Seattle Mariners | Seattle Mariners (MLB) | Traded from Yankees with Danny Farquhar to Mariners for Ichiro Suzuki |
| Ichiro Suzuki | lf-rf | 38.273 | New York Yankees | New York Yankees (MLB) | Traded from Mariners to Yankees for D.J. Mitchell and Danny Farquhar |
| Rob Brantly | C | 23.009 | Miami Marlins | New Orleans (AAA) | Traded from Tigers with Jacob Turner and Brian Flynn to Marlins for Anibal Sanchez and Omar Infante |
| Brian Flynn | P | 22.095 | Miami Marlins | Jacksonville (AA) | Traded from Tigers with Jacob Turner and Rob Brantly to Marlins for Anibal Sanchez and Omar Infante |
| Danny Farquhar | P | 25.156 | Seattle Mariners | Tacoma (AAA) | Traded from Yankees with D.J. Mitchell to Mariners for Ichiro Suzuki |
| Kelvin De La Cruz | P | 23.356 | Detroit Tigers | Erie (AA) | Designated for assignment |
| Jonnathan Aristil | P | 25.235 | - | | Released by Astros |
| Mitch Moreland | 1B | 26.320 | Texas Rangers | Round Rock (AAA) | Assigned to Round Rock (AAA) for rehab |
| Andrew Carraway | P | 25.322 | Seattle Mariners | Tacoma (AAA) | DL-7 Injured |
| Paul Phillips | C | 35.099 | Toronto Blue Jays | Las Vegas (AAA) | DL-7 Injured |
| Luis Hurtado | C | 23.261 | Toronto Blue Jays | Las Vegas (AAA) | Activated from DL |
| Fabio Castillo | P | 23.154 | Texas Rangers | Round Rock (AAA) | Activated from DL |
| James Skelton | C | 26.268 | Washington Nationals | Syracuse (AAA) | Activated from DL |
| Cale Iorg | SS | 26.320 | Detroit Tigers | Toledo (AAA) | Activated from DL |
| Les Walrond | P | 35.258 | - | | Released by Blue Jays |
| Rob Gilliam | P | 24.237 | Washington Nationals | Harrisburg (AA) | DL-7 Injured |
| Aaron Baker | 1B | 24.317 | Baltimore Orioles | Bowie (AA) | DL-7 Injured |
| Scott Schneider | P | 24.046 | - | | Released by Cardinals |
| Kevin Nolan | SS-1B | 24.223 | Toronto Blue Jays | Dunedin (A+) | DL-7 Injured |
| Jared Potts | P | 27.019 | Toronto Blue Jays | Dunedin (A+) | DL-7 Injured |
| Eswarlin Jimenez | P | 20.239 | Los Angeles Angels | Cedar Rapids (A) | Activated from DL |
| David Rollins | P | 22.214 | Houston Astros | Lexington (A) | DL-7 Injured |
| Brett Newsome | 1B | 25.333 | Washington Nationals | Hagerstown (A) | DL-7 Injured |
| Enrico Jimenez | P | 23.166 | Baltimore Orioles | Aberdeen (A-) | Suspended |
| Frank Francisco | P | 32.042 | New York Mets | Brooklyn (A-) | Assigned to Brooklyn (A-) for rehab |
| Mike Baxter | 1B | 27.228 | New York Mets | Buffalo (AAA) | Assigned to Buffalo (AAA) for rehab |
| Eduardo Nunez | SS | 25.038 | New York Yankees | Tampa (A+) | Assigned to Tampa (A+) |
| Jonathan Roof | LF-2B | 23.181 | San Diego Padres | Eugene (A-) | Assigned to Eugene (A-) |
| Austin Nola | SS | 22.207 | Miami Marlins | Jamestown (A-) | Assigned to Jamestown (A-) |
| Austin Nola | SS | 22.207 | Miami Marlins | Greensboro (A) | Assigned to Greensboro (A) |
| John Ruettiger | CF | 22.305 | Baltimore Orioles | Bowie (AA) | Assigned to Bowie (AA) |
| Cole Leonida | C | 23.210 | Washington Nationals | Gulf Coast Nationals (Rk) | Assigned to Gulf Coast Nationals (Rk) for rehab |
| Ben Heath | C | 23.289 | Houston Astros | Corpus Christi (AA) | Assigned to Corpus Christi (AA) |
| Richie Shaffer | 3B | 21.130 | Tampa Bay Rays | Hudson Valley (A-) | Assigned to Hudson Valley (A-) |
| Tyler Austin | P | 20.321 | New York Yankees | Gulf Coast Yankees (Rk) | Assigned to Gulf Coast Yankees (Rk) |
| Julio Concepcion | LF | 22.321 | New York Mets | Kingsport (Rk) | Assigned to Kingsport (Rk) |
| Henry Moreno | 1B | 23.047 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Angelberth Montilla | RF | 23.103 | Washington Nationals | Hagerstown (A) | Assigned to Hagerstown (A) |
| Angelberth Montilla | RF | 23.103 | Washington Nationals | Auburn (A-) | Assigned to Auburn (A-) |
| Gavi Nivar | LF-CF | 22.310 | Pittsburgh Pirates | State College (A-) | Assigned to State College (A-) |
| Carlos Alvarez | 2B-3B | 26.240 | Washington Nationals | Auburn (A-) | Assigned to Auburn (A-) |
| Roderick Bernadina | RF | 19.347 | Baltimore Orioles | Frederick (A+) | Assigned to Frederick (A+) |
| Jorge Rivero | 2B | 23.198 | New York Mets | Kingsport (Rk) | Assigned to Kingsport (Rk) |
| Zach Larson | OF | 18.288 | Minnesota Twins | Gulf Coast Twins (Rk) | |
| Raymond Hunnicutt | OF | 18.218 | Baltimore Orioles | Gulf Coast Orioles (Rk) | |
| Josh Almonte | OF | 18.176 | Toronto Blue Jays | Gulf Coast Blue Jays (Rk) | Assigned to GCL Blue Jays (Rk) |
| Zachary Livingston | C | 22.296 | Los Angeles Angels | Orem (Rk) | Assigned to Orem (Rk) |
| Harold Ramirez | | 17.320 | Pittsburgh Pirates | Gulf Coast Pirates (Rk) | |
| Francisco Rosario | | 22.011 | New York Yankees | Gulf Coast Yankees (Rk) | |
| Adonis Garcia | | 27.102 | New York Yankees | Tampa (A+) | |
| Mike Ojala | P | 24.334 | Miami Marlins | Jupiter (A+) | Assigned to Jupiter (A+) |
| Rafael Perez | P | 30.069 | Cleveland Indians | Akron (AA) | Assigned to Akron (AA) for rehab |
| Ethan Hollingsworth | P | 25.080 | Kansas City Royals | Northwest Arkansas (AA) | Assigned to Northwest Arkansas (AA) |
| Taylor Jordan | P | 23.187 | Washington Nationals | Auburn (A-) | Assigned to Auburn (A-) |
| Peter Birdwell | P | 25.170 | New York Mets | Kingsport (Rk) | |
| William Scott | P | 21.324 | San Diego Padres | Tucson (AAA) | Assigned to Tucson (AAA) |
| Casey Kelly | P | 22.292 | San Diego Padres | Arizona Padres (Rk) | |
| Chris Haney | P | 23.160 | San Diego Padres | Tucson (AAA) | Assigned to Tucson (AAA) |
| Alex Wimmers | P | 23.264 | Minnesota Twins | Gulf Coast Twins (Rk) | |
| Ben Tomchick | P | 22.218 | Kansas City Royals | Burlington (Rk) | |
| Jake Boyd | P | 22.198 | Los Angeles Angels | Orem (Rk) | Assigned to Orem (Rk) |
| Patrick Scoggin | P | 21.152 | Atlanta Braves | Gulf Coast Braves (Rk) | |
| Eric Semmelhack | P | 21.197 | Milwaukee Brewers | Wisconsin (A) | Assigned to Wisconsin (A) |
| Daniel Camarena | P | 19.256 | New York Yankees | Gulf Coast Yankees (Rk) | |
| Andrew Stueve | P | 23.107 | Kansas City Royals | Burlington (Rk) | Assigned to Burlington (Rk) |
| Austin Fairchild | P | 18.120 | Kansas City Royals | Burlington (Rk) | |
| Chris Flexen | P | 18.022 | New York Mets | Kingsport (Rk) | |
| Blake Schwartz | P | 22.287 | Washington Nationals | Hagerstown (A) | Assigned to Hagerstown (A) |
| Mitch Mustain | | 24.147 | Chicago White Sox | Kannapolis (A) | Assigned to Kannapolis (A) |
| Dioni Dominguez | | 21.276 | Baltimore Orioles | Gulf Coast Orioles (Rk) | |
| Trey Lang | | 20.066 | Chicago Cubs | Arizona Cubs (Rk) | |
| Mark Hardy | P | 24.081 | San Diego Padres | Eugene (A-) | Assigned to Eugene (A-) |
